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Recent papers on concentration dose cell concentrations viability

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Effect of <i>P. cattleianum</i> HE on the viability of MCF-7 breast cancer and HCT-116 colorectal cancer cells in MTT assay. (A) Dose-response curve showing percent cell viability versus extract concentration (μg/mL), (B) Representative images of the treated cancer cells by HE compared to control.
2025 · Figshare · (14571719), Eman M. El-Deeb, (9276104), Fatma A. Moharram, (14571722), Hussein S. Taha et al.
2025
Dose- and time-dependent effect of high glucose concentration on viability of notochordal cells and expression of matrix degrading and fibrotic enzymes
2013 · International Orthopaedics · Park, Eun‐Young, Park, Jong-Beon
2013
Weight-based dosing of calcineurin inhibitors in patients with obesity undergoing hematopoietic stem cell transplantation: Blood concentrations and clinical outcomes
2026 · Transplantation and Cellular Therapy · Nakagaki, Midori, McIlwain, Jessica, Henden, Andrea et al.
2026
Background: Platelet transfusions are essential in the management of thrombocytopenia, bleeding disorders, and hematologic malignancies. With cannabis use rising worldwide, its impact on donor platelet quality and transfusion efficacy remains poorly understood. This study investigated the effects of cannabis joint extracts (CJE) on platelet activation, pro-coagulant phenotype, mitochondrial function, and cytokine/chemokine release, with implications for transfusion safety. Methods: Human platelets were exposed in vitro to increasing concentrations of two CJE with distinct cannabinoid profiles: Orchid (O-CJE, THC 10.4%, CBD 14.7%) and QCGold (G-CJE, THC 25.5%, CBD 0.04%). Platelet activation (CD62P, Annexin V), mitochondrial depolarization, ATP levels, aggregation responses, and cytokine secretion (CCL3, PF4) were assessed. RBC lysate experiments were performed to assess the role of hemolysis. The involvement of CB1 and CB2 receptors was tested using specific antagonists, and activation of p38 MAPK and NF-kB pathways was evaluated. Functional effects of platelet supernatants were examined on EA.hy926 endothelial cells. Results: CJE exposure induced dose-dependent platelet activation, characterized by increased CD62P expression, Annexin V binding, mitochondrial depolarization, and ATP depletion, consistent with metabolic stress. Platelet aggregation in response to ADP, collagen, and arachidonic acid was impaired, suggesting a pre-activated or refractory phenotype. RBC lysate did not reproduce the observed effects, indicating hemolysis is unlikely to be the underlying mechanism. CBR1 and CBR2 antagonists did not attenuate platelet activation, while signalling analysis revealed activation of p38 MAPK and NF-kB pathways. Exploratory proteomics indicated modulation of proteins involved in angiogenesis, cytoskeletal organization, and stress responses. Elevated plasma levels of CCL3 and PF4 and endothelial activation (IL-6 secretion, CD54, CD62P, CD62E expression) further suggested a pro-inflammatory environment. Conclusions: Cannabis exposure can alter platelet phenotype and signalling under in vitro conditions, potentially affecting platelet function and interactions with the vascular endothelium. However, these findings require confirmation in vivo to determine their clinical relevance. Future studies should aim to establish exposure thresholds and clarify whether cannabis use has implications for transfusion safety, given potential risks of reduced efficacy or increased thrombotic complications in recipients.
2026 · Open MIND · Loubaki, Lionel
2026
Observed steady-state tenofovir diphosphate (TFV-DP) concentrations in peripheral blood mononuclear cells (PBMCs) by dosing arm.
2025 · Figshare · (7339643), Kenneth K. Mugwanya, (18618004), Matilda Saina, (7901144), Nelly R. Mugo et al.
2025
STZ dose-dependent effect on insulin function in adhered monolayer islets. The efficacy of average β-cell (so-called “endocrine power”) was evaluated as the amount of insulin produced per average β-cell per hour when stimulated with a high 25 mM glucose concentration. It was measured as the amount of insulin produced by all islets (stimulated) within a well of the culture plate (concentration multiplied by volume), divided by the number of β-cells in the well (Fig 6B), and incubation time (one hour). No significant differences among groups were observed.
2025 · Figshare · (485311), Makiko Kumagai-Braesch, (130901), Ming Yao, (21474419), Nils Ågren et al.
2025
Patient-specific pharmacological and clinical parameters evaluated from model fits. A–D Within the patient level, the fold change in IL-6 concentration (baseline to peak) cannot be predicted from a single indicator, such as total CAR-T dose, initial tumor burden, CAR-T cell fold change (dose to peak), or time of IL-6 peak. E However, for the majority of patients, the model predicts that the peak of CAR-T cells occurs between 0 and 5 days after the cytokine peak (gray area). F For all patients, the cytokine peak is predicted to occur between 0 and 2 days after the peak of activated macrophages (gray area). G,H The tumor burden at days 28 and 90 post-therapy initiation shows a weak negative correlation with CAR-T cell fold change (a value of 0.1 cells is assigned for cases where the model predicts zero tumor cells). I The model predicts a moderate correlation between tumor burden responses at days 28 and 90. In each panel, the best linear fit for the red points is shown in light red,
2025 · Figshare · (21030812), Daniela S Santurio, (21030815), Luciana R C Barros, (261610), Ingmar Glauche et al.
2025
Flow-through cell dissolution method as predictor of sulfamethoxazole and trimethoprim plasma concentrations: studies using two-dose formulations
2025 · International Journal of Research in Pharmaceutical Sciences · López, José Raúl Medina, Ríos-López, A, Hurtado, Marcela et al.
2025
Concomitant letermovir affects the optimal concentration-to-dose ratio of tacrolimus after switching from intravenous to oral tacrolimus administration in hematopoietic cell transplantation patients receiving fluconazole prophylaxis
2025 · International Journal of Hematology · Nakashima, Toshihisa, Inamoto, Yoshihiro, Ito, Ayumu et al.
2025
The impact of <i>CYP3A5</i> , <i>NR1I2</i> , and <i>POR</i> polymorphisms on tacrolimus dose-adjusted concentration and clinical outcomes in adult allogeneic haematopoietic stem cell transplantation
2025 · Xenobiotica · Gao, Yuan, Ma, Jingjing
2025
#De Novo Longterm-Release TacrolimusEnhances T Cell Suppression In Patients With Low Concentration-To-Dose Ratios After Kidney Transplantation – A Matched Cohort Analysis
2025 · Simon, Aberger,, Max, Schuller,, Anna, Mooslechner, Agnes et al.
2025
Objective: To investigate the use of biological ensiling of organic residues generated by pectiniculture as a nutritive medium in the production of the microalgae Tetraselmis suecica. Theoretical Framework: Biological ensiling is a process that transforms organic residues into a nutritive source through fermentation. In this context, pectiniculture residues, specifically the soft parts of fan shells, can be recycled and used as alternative media. Microalgae, such as T. suecica, are photosynthetic organisms that require nutrients for their growth, and their cultivation can benefit from these residues. Method: Twelve vertical bioreactors were used with three experimental treatments (T1, T2, T3) and treatments, one control (CT), with three replicates per treatment. The microalgae were inoculated at a density of 70 x 104 cells mL-1 in a culture volume of 80 L. Cultures were grown in open air with constant aeration. 0-, 60-, 70-, and 80-mL L-1 of the biological silage of fan shell soft parts (EBCA) were dosed. The silage was also chemically characterized and the growth of the microalgae and its relationship with temperature, salinity and pH were determined. Results and Discussion: The results showed that the dosage of 70 mL L-1 of EBCA in T. suecica cultures favors the growth of the microalgae. A significant increase in biomass and cell density was observed compared to the other treatments, indicating that this concentration of silage provides a suitable nutrient medium. The study also analyzed how temperature, salinity and pH conditions affect the growth of T. suecica, finding that certain optimal ranges of these factors can maximize biomass production. Research Implications: Our study demonstrates that pectin culture waste can be effectively recycled as a culture medium for T. suecica production, offering a sustainable and environmentally friendly solution for waste management. This could reduce reliance on traditional culture media and promote more sustainable practices in aquaculture and the microalgal industry. Originality/Value: This study stands out for its innovative approach in the use of organic waste from pectiniculture, such as the soft parts of the fan shell, for the generation of an alternative nutrient medium for microalgae. In addition, large volumes of fan shell wastes are currently being generated, so our work contributes to the management of these wastes.
2024 · Revista de Gestão Social e Ambiental · Espinoza, Sorayda Mendoza, Vigo, Luis Campoverde, Tuanama, Karoly Rossmery Sagastegui et al.
2024
Anticancer effects of high-dose extracellular citrate treatment in pancreatic cancer cells under different glucose concentrations
2024 · Heliyon · Kim, Wonjin, Park, Sanghee, Park, Tae-Hyun et al.
2024
Intratumoral Chemotherapy: The Effects of Drug Concentration and Dose Apportioning on Tumor Cell Injury
2024 · Bioengineering · Warner, J.S., Kinsey, C. Matthew, Bates, Jason H. T. et al.
2024
Engineered nanomaterials exert sublethal bacterial stress at very low doses: Effects of concentration, light, and media on cell membrane permeability
2024 · The Science of The Total Environment · Wu, Shushan, Wells, George, Gray, Kimberly A.
2024
Posaconazole in paediatric malignancy and haematopoietic stem cell transplant: dosing to achieve therapeutic concentration
2024 · Journal of Antimicrobial Chemotherapy · Weerdenburg, Heather, Walker, Hannah, Curtis, Nigel et al.
2024
Pharmacogenetic and clinical predictors of voriconazole concentration in hematopoietic stem cell transplant recipients receiving CYP2C19-guided dosing
2023 · The Pharmacogenomics Journal · Patel, Jai N., Robinson, Myra, Morris, Sarah et al.
2023
Small volume bone marrow aspirates with high progenitor cell concentrations maximize cell therapy dose manufacture and substantially reduce donor hemoglobin loss
2023 · BMC Medicine · Epah, Jeremy, Spohn, Gabriele, Preiß, Kathrin et al.
2023
Dose metric evaluation of a cell-based bioassay for assessing the toxicity of metals to Dugong dugon: Effect of metal-media interactions on exposure concentrations
2023 · Aquatic Toxicology · Bailey, David J., Finlayson, Kimberly A., Doğruer, Gülşah et al.
2023
Impact of Early Intrapatient Variability of Tacrolimus Concentrations on the Risk of Graft-Versus-Host Disease after Allogeneic Stem Cell Transplantation Using High-Dose Post-Transplant Cyclophosphamide
2022 · Pharmaceuticals · Marco, Daniel N., Salas, María Queralt, Gutiérrez, Gonzalo et al.
2022
Can First-Dose Therapeutic Drug Monitoring Predict the Steady State Area Under the Blood Concentration-Time Curve of Busulfan in Pediatric Patients Undergoing Hematopoietic Stem Cell Transplantation?
2022 · Frontiers in Pediatrics · Alsultan, Abdullah, Albassam, Ahmed A., Alturki, Abdullah et al.
2022
Pharmacological Dose-Effect Profiles of Various Concentrations of Humanised Primary Bile Acid in Encapsulated Cells
2022 · Nanomaterials · Mooranian, Armin, Jones, Melissa, Walker, Daniel P. et al.
2022
Methylene blue and photodynamic therapy for melanomas: Inducing different rates of cell death (necrosis and apoptosis) in B16-F10 melanoma cells according to methylene blue concentration and energy dose
2021 · Photodiagnosis and Photodynamic Therapy · Grande, Murilo Penteado Del, Miyake, Andréa Midory, Nagamine, Márcia Kazumi et al.
2021
Platelet rich plasma concentration improves biologic mesh incorporation and decreases multinucleated giant cells in a dose dependent fashion
2021 · Journal of Tissue Engineering and Regenerative Medicine · Araujo‐Gutierrez, Raquel, Eps, Jeffrey L. Van, Scherba, Jacob C. et al.
2021
Repeat and single dose administration of gadodiamide to rats to investigate concentration and location of gadolinium and the cell ultrastructure
2021 · Scientific Reports · Davies, Julie, Marino, Michael, Smith, Adrian et al.
2021
Application of Brunauer–Emmett–Teller (BET) theory and the Guggenheim–Anderson–de Boer (GAB) equation for concentration-dependent, non-saturable cell–cell interaction dose-responses
2020 · Journal of Pharmacokinetics and Pharmacodynamics · Nguyen, Van Anh, Ramanathan, Murali
2020
High-Dose Neonatal Vitamin A Supplementation to Bangladeshi Infants Increases the Percentage of CCR9-Positive Treg Cells in Infants with Lower Birthweight in Early Infancy, and Decreases Plasma sCD14 Concentration and the Prevalence of Vitamin A Deficiency at Two Years of Age
2020 · Journal of Nutrition · Ahmad, Shaikh Meshbahuddin, Huda, M. Nazmul, Raqib, Rubhana et al.
2020
Platelet Lysate as source of growth factors for Bone Marrow derived Mesenchymal Stromal cells clinical expansion: a study of starting platelet concentration dose-dependent effects
2020 · Cytotherapy · Becherucci, Valentina, Nisticò, F., Piccini, Luisa et al.
2020
Individualized dosing schedule of sunitinib switched through plasma concentration monitoring in patients with metastatic renal cell carcinoma.
2020 · Journal of Clinical Oncology · Zhu, Xudong, Zhang, Xingming, Zhang, Haoran et al.
2020