cell lines cells cancer primary

The gap

Supporting evidence — 7 representative gaps

• A novel combination therapy for ER+ breast cancer suppresses drug resistance via an evolutionary double-bind (2026) · doi

  The study used only two breast cancer cell lines (T-47D and MCF-7); validation across additional ER+ breast cancer models would strengthen generalizability.

  Keywords: breast cancer used cell lines validation across additional models strengthen generalizability
• EXPLORING THE PHARMACOLOGICAL POTENTIAL OF SYNECHOCOCCUS SP. PGDR2 THROUGH PHYTOCHEMICAL AND IN VITRO BIOACTIVITY STUDIES (2026) · doi

  The study evaluated bioactivity only against MG-63 osteosarcoma cells; testing against other cancer cell lines would determine the breadth of anticancer potential.

  Keywords: against evaluated bioactivity osteosarcoma cells testing cancer cell lines determine breadth anticancer potential
• Oral supplementation of sodium butyrate prevents lipid metabolism disorders and intestinal injury by modulating immunity, intestinal barrier functions, and gut microbiota in a corticosterone-induced chronic stress model in mice (2026) · doi

  Only the IEC-6 cell line was used for in vitro studies; testing with primary intestinal epithelial cells or other cell lines would strengthen the generalizability of results.

  Keywords: cell line used vitro testing primary intestinal epithelial cells lines strengthen generalizability
• Reinvigorating COTL1high NK cells via GITR signalling overcomes immune checkpoint blockade resistance in tsMHC-I-impaired tumours (2026) · doi

  The study uses only three hepatoma cell lines (H22, Hep-53.4, and Hepa1-6) and two mouse strains (BALB/c and C57BL/6) for in vivo validation; the generalizability of GITR signaling-mediated COTL1high NK cell reinvigoration to other tumor types beyond hepatocellular carcinoma and across diverse genetic backgrounds requires investigation with additional syngeneic tumor models and immunocompetent mouse strains.

  Keywords: GITR signaling COTL1 NK cells hepatocellular carcinoma syngeneic tumor models immunocompetent
• Shared Plant-human Biology: Herbicide Effects and New Biomarkers Perspectives (2026) · doi

  The connection between glufosinate inhibition of glutamine synthetase and glutamate excitotoxicity-mediated neurodegeneration requires mechanistic validation using primary human neurons and astrocyte co-cultures exposed to herbicide-relevant concentrations, with concurrent measurement of glutamate/glutamine ratios and oxidative stress markers to establish causality beyond isolated cell lines.

  Keywords: glufosinate glutamine synthetase glutamate excitotoxicity primary neurons astrocyte oxidative stress
• DNA Methyltransferase Inhibitors in Triple-Negative Breast Cancer: Mechanisms, Limitations, and Therapeutic Potential (2026) · doi

  The correlation between DNMT1 expression and ERα methylation status has been established in breast cancer cell lines, but the specific mechanisms by which DNMT1 activates the ERα promoter in triple-negative breast cancer cells remain unclear. Future studies should investigate the direct binding sites of DNMT1 on the ERα gene regulatory regions and characterize the epigenetic crosstalk between DNA methyltransferase inhibitors and estrogen receptor signaling in TNBC.

  Keywords: DNMT1 ERα methylation triple-negative breast cancer promoter binding DNA methyltransferase inhibitors
• Tumor growth inhibitory activity of the P2X7 receptor antagonist AZ10606120 in two cell lines of human glioblastoma (2026) · doi

  The study was limited to two glioblastoma cell lines (U87MG and T98G); validation in additional cell lines and primary tumor models is needed.

  Keywords: cell lines limited glioblastoma validation additional primary tumor models needed