temperature threshold thresholds strengthen rare

The gap

Supporting evidence — 2 representative gaps

• Temperature dependent immunological responses of Spoladea recurvalis exposed to entomopathogenic fungi (2026) · doi

  The sequencing data were analyzed using EPI2ME Desktop agent software (3.7.3) with a 0.5% abundance threshold for bacterial classification, but the sensitivity, specificity, and classification confidence thresholds of this bioinformatics pipeline for detecting rare bacterial taxa associated with entomopathogenic fungal infection are not discussed. Validation of the microbiome findings using alternative 16S rRNA analysis pipelines (e.g., QIIME2, mothur) would strengthen the robustness of bacterial community results across temperature conditions.

  Keywords: 16S rRNA sequencing EPI2ME bioinformatics classification threshold microbiome entomopathogenic fungi
• Response strategies of Ochroma lagopus to short-term low-temperature stress and rewarming: a photosynthesis-antioxidant-carbon network analysis (2026) · doi

  The specific thresholds (7°C, 5°C, 3°C) identified may be species-specific; testing whether these temperature threshold effects apply to other tropical tree species would strengthen ecological applicability.

  Keywords: specific species thresholds identified testing whether temperature threshold effects apply tropical tree strengthen ecological applicability