Can be conducted to develop more effective extraction

The gap

Evidence profile

Sourced from the stated research gap and future-work section and recommendations and discussion of the source papers, classified as general, spanning 6 journals.

Supporting evidence — 8 representative gaps

• Phytochemical Extraction from Mediterranean Plants: A Comparative Study of Traditional Techniques (2026) · Sciences of Phytochemistry · doi

  The lack of studies on the effect of solvent polarity and extraction technique on the recovery of bioactive compounds from Portuguese native plants. The need for a comparative study on the extraction yields and phytochemical fingerprints of different solvents and extraction techniques.

  generalstated research gap
  Keywords: lack studies effect solvent polarity extraction technique recovery
• Phytochemical Extraction from Mediterranean Plants: A Comparative Study of Traditional Techniques (2026) · Sciences of Phytochemistry · doi

  Future studies should incorporate replicated experiments, standardized sample preparation, and quantitative targeted phytochemical analysis. Future studies should investigate the effect of other extraction techniques and solvents on the recovery of bioactive compounds. Future studies should develop new products or applications using these plants.

  generalfuture-work section
  Keywords: future studies incorporate replicated experiments standardized sample preparation
• Acetone-Based Comparative Phytochemical Profiling of Indonesian Ethnomedicinal Plants: Effects of Maceration and Soxhlet Extraction (2026) · JURNAL SURYA TEKNIKA · doi

  (a) compound quantification via HPLC-DAD and LC-MS/MS; (b) validated in vitro bioactivity assays (DPPH, MIC, MTT assay); (c) bioactivity-guided fractionation; (d) OECD 423 acute toxicity testing before formulation development. include REFERENCES H. J. Woerdenbag and O. Kayser, "Jamu: Indonesian traditional rational phytopharmacological use," J. Herb. Med., vol. 4, no. 2, pp. 51–73, 2014. herbal medicine towards N. N. Azwanida, "A review on the extraction methods used in medicinal plants: principle, strength and limitation," Med. Aromat. Plants, vol. 4, no. 196, 2015. M. D. L. De Castro and F. Priego-Capote, "Soxhlet extraction: Past and present panacea," J. Chromatogr. A, vol. 1217, no. 16, pp. 2383–2389, 2010. S. Sun et al., "Impact of extraction techniques on phytochemical composition and bioactivity of natural product mixtures," Front. Pharmacol., vol. 16, art. 1615338, 2025. L. G. M. da Silva et al., "Synergy between solvent polarity and composition for efficient extraction of bioactive compounds from pitaya," Food Mater. Res., vol. 5, no. 1, 2025. M. Naczk and F. Shahidi, "Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis," J. Pharm. Biomed. Anal., vol. 41, no. 5, pp. 1523– 1542, 2006. C. Reichardt and T. Welton, Solvents and Solvent Effects in Organic Chemistry. Hoboken, NJ, USA: John Wiley & Sons, 2011. A. J. Harborne, Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Dordrecht, Netherlands: Springer Science & Business Media, 1998. W. C. Evans, Trease and Evans Pharmacognosy, 15th ed. India: Saunders/Elsevier Science, 2002. A. E. Sofowara, Medicinal Plants and Traditional Medicine in Africa, vol. 2. Ibadan, Nigeria: Spectrum Books Ltd., 1993. K. S. Sim, A. M. Sri Nurestri, and A. W. Norhanom, "Phenolic content and antioxidant activity of crude and fractionated extracts of Pereskia bleo (Kunth) DC. (Cactaceae)," African J. Pharm. Pharmacol., vol. 4, no. 5, pp. 193–201, 2010. S. Zareisedehizadeh, C.-H. Tan, and H.-L. Koh, "A review of botanical characteristics, traditional usage, chemical components, pharmacological activities, and safety of Pereskia bleo (Kunth) DC.," Evidence-Based Complement. Altern. Med., vol. 2014, art. 326107, 2014. R. M. P. Gutierrez, S. Mitchell, and R. V. Solis, "Psidium guajava: A review of its traditional uses, phytochemistry J. Ethnopharmacol., vol. 117, no. 1, pp. 1–27, 2008. pharmacology," and Z. Khan, D. Shahwar, M. K. Y. Ansari, and R. Chandel, "Toxicity assessment of anatase (TiO2) nanoparticles: A pilot study on stress response alterations and DNA damage studies in Lens culinaris Medik.," Heliyon, 2019. I. T. Fu’adah, S. A. Sumiwi, and G.

  generalrecommendationsevidence 5/5
  Keywords: extraction traditional bioactivity review plants toxicity medicine medicinal techniques phytochemical composition pharmacol solvent pharm science
• DETERMINE THE BEST CONDITIONS FOR EXTRACTING BIOATIVE COMPOUNDS FROM ROSMARINUS OFFICINALIS LEAVES (2026) · Zenodo (CERN European Organization for Nuclear Research) · doi

  Further studies can be conducted to develop more effective extraction methods for bioactive compounds. The biological activity of extracts can be tested against other types of pathogens.

  generalfuture-work sectionevidence 5/5
  Keywords: further studies conducted develop effective extraction methods bioactive
• Effect of different solvent on phytochemical content of Moringa oleifera L. leaves. (2026) · International Journal of Life Science Research Archive · doi

  Further studies can be conducted to evaluate the antioxidant activity of the extracted phytochemicals. The study's findings can be used to develop effective extraction methods for phytochemicals from Moringa oleifera L. leaves.

  generalfuture-work sectionevidence 5/5
  Keywords: further studies conducted evaluate antioxidant activity extracted phytochemicals
• Determination of essential oil composition of Laurencia obtusa and Ceramium spp. in Black Sea and antileishmanial activity (2026) · International Journal of Secondary Metabolite · doi

  Further research is needed to confirm the findings and to evaluate the in vivo efficacy of the extracts. The study suggests the potential for controlled experiments in laboratory animals to assess MIC values and to optimize dosage for therapeutic use. The study implies that future research should focus on correlation-based evaluations using isolated compounds, detailed cytotoxicity analyses, and comprehensive in vivo assessments.

  generalfuture-work sectionevidence 5/5
  Keywords: further research needed confirm findings evaluate vivo efficacy
• The analysis of sodium butyrate content in various dietary supplements available on the Polish market (2026) · Annales Academiae Medicae Silesiensis · doi

  Due to the unique nature of the method used, data on the potential impact of these substances on the extraction efficiency and chromatography result are limited. Further research in this area is warranted to enhance the quality control and safety of dietary supplements.

  generaldiscussionevidence 4/5
  Keywords: unique nature used potential impact substances extraction efficiency chromatography result limited further area warranted enhance
• Isolation and characterization of caffeic acid from Cobra Lily (Arisaema propinquum) and evaluation of its biological activities (2026) · International Journal of Secondary Metabolite · doi

  Further research is needed to investigate the molecular targets and pathways of the herb's antioxidant and antimicrobial activities. Further research is needed to evaluate the toxicity of the herb's extracts. Further research is needed to develop standardized plant-based therapeutics from the herb's extracts.

  generalfuture-work sectionevidence 4/5
  Keywords: further research needed investigate molecular targets pathways herb

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