Plant synthetic biology requires building intellectual and physical infrastructure to rapidly design and assemble synthetic genetic systems in plants

The gap

Evidence profile

Sourced from the open questions and future work of the source papers, classified as methodology gap, drawn from work published between 2016 and 2017, spanning 2 journals. Those papers have been cited 11 times in total.

Supporting evidence — 4 representative gaps

• Synthetic Biology (2016) · Synthetic Biology · cited 3× · doi

  The paper identifies a 'daunting knowledge gap' between constructing synthetic biological systems and understanding them, but does not specify empirical methods to measure or close this gap. Future work should develop quantitative frameworks to compare synthetic bottom-up design approaches versus classical knock-out methodologies in determining gene contribution to cellular function.

  methodology gapopen questionsevidence 5/5
  Keywords: synthetic biology bottom-up design knock-out methodology gene contribution cellular function
• Synthetic Biology (2016) · Synthetic Biology · cited 3× · doi

  The author argues that design criteria in synthetic biology incorporate aesthetic and economic norms alongside scientific criteria, but does not propose methods to systematically identify and measure these hybrid value conglomerations. A phenomenology of values framework must be developed and empirically tested across specific synthetic biology design practices.

  methodology gapfuture workevidence 5/5
  Keywords: synthetic biology design values aesthetic norms economic values hybrid practices normative analysis
• Production biology (2016) · Synthetic Biology · doi

  While the paper argues that tinkering production methodology should incorporate trial-and-error approaches for synthetic biology, there is no formalized framework specifying when iterative tinkering should cease, what constitutes sufficient empirical validation of engineered biological constructs, or how to distinguish between productive tinkering and inefficient experimentation in production biology contexts.

  methodology gapopen questionsevidence 5/5
  Keywords: tinkering production trial-and-error synthetic biology validation engineered constructs
• Editorial overview: Synthetic biology: Frontiers in synthetic biology (2017) · Current Opinion in Chemical Biology · cited 5× · doi

  Plant synthetic biology requires building intellectual and physical infrastructure to rapidly design and assemble synthetic genetic systems in plants, but the paper does not specify what standardized assembly methods, plant-specific biobrick libraries, or transformation protocols should be prioritized for this infrastructure development.

  methodology gapfuture workevidence 5/5
  Keywords: plant synthetic biology DNA assembly genetic systems infrastructure standardization

Questions about this gap