Open research questions in Biosensors and Analytical Detection
222 gap statements mined from Biosensors and Analytical Detection papers in our 4.5M-paper local library, which holds 2,026 papers on the topic — drawn mostly from each paper's own stated research gap, future-work, challenge and limitation notes, and its abstract. The ones listed below are a selection still marked open; each names the study that raised it, with a DOI link where the paper has one.
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What the literature leaves open
The need to reduce costs and accelerate development of point-of-care diagnostics. The challenge of producing diagnostics that can be used in resource-limited settings. The difficulty of detecting disease biomarkers with high sensitivity and specificity using low-cost assays.
The study does not provide a comprehensive comparison with other diagnostic methods. The assay's performance in real-world settings is not evaluated. The study relies on a limited number of experiments and samples.
The lack of a portable and rapid nucleic acid detection platform for point-of-care testing. The limitations of current RT-qPCR instruments in terms of size, complexity, and operational requirements. The need for a unified microfluidic device that integrates multiple functional components for nucleic acid detection.
A Portable Dual-Mode Microfluidic Device Integrating RT-qPCR and RT-LAMP for Rapid Nucleic Acid Detection in Point-of-Care Testing · 2026 · DOIConventional molecular assays are limited by their dependence on sophisticated instrumentation and trained personnel. There is a need for rapid, reliable, and cost-effective detection methods for GM-derived ingredients. Existing methods do not address the need for on-site GMO screening.
Development of a Gold Nanoparticle-Based Amplification-Free Nanobiosensor for Rapid DNA Detection Supported by Machine Learning · 2026 · DOISignal-encoding limitations, including the need for high-dimensional detection and accurate measurement of multiple analytes. Computational limitations, including the need for strong methods to minimize errors that come from measuring multiple analytes.
Evolution of Next-Generation Multiplex Lateral Flow Immunoassays: From Engineered Nanomaterials to AI-Driven Detection · 2026 · DOITraditional gold nanoparticles-labeled lateral flow assays have insufficient signal strength when detecting nucleic acids. The need for rapid, sensitive, and user-friendly pathogen detection methods. The lack of efficient and easy-to-operate solutions for the visual detection of foodborne pathogens.
Polystyrene Microsphere-Labeled Lateral Flow Assay for the Visual Detection of Foodborne Pathogens · 2026 · DOIThe paper identifies the need for robust synthetic recognition elements for point-of-care nucleic acid tests. It also mentions the challenge of minimizing non-specific background signals in paper-based assays. The paper notes the importance of optimizing the components of the NAVF platform to improve sensitivity and reduce non-specific adsorption.
Molecularly Imprinted Polymers as Biomimetic Test Zones in Paper-Based Nucleic Acid Assays—Comparing Vertical and Lateral Flow Formats · 2026 · DOIThe need to optimize the conditions for the colorimetric sensor to exhibit a linear response toward phenylephrine. The potential interference from common interferents such as ascorbic acid, dopamine, uric acid, and glucose. The requirement for a stable and reliable sensing platform.
Selective Colorimetric Determination of Phenylephrine Using a Prussian Blue Nanoparticle-Modified Paper-Based Sensor · 2026 · DOIConventional methods are labor-intensive, time-consuming, and require a laboratory setting. Existing diagnostic approaches often fail to meet the speed and accessibility requirements for field deployment.
Portable Dual-Mode Biosensor for Quantitative Determination of Salmonella in Lateral Flow Assays Using Machine Learning and Smartphone-Assisted Operation · 2026 · DOIThe limited effectiveness of optical methods in detecting infectious particles.
The platform may not be suitable for decentralized or resource-limited settings due to infrastructure requirements. The study only tested 80 specimens collected from CKD patients.
Rapid Finger-Pump Microfluidic Paper-Based Assay Platform for Monitoring Calcium Ions in Human Biofluids · 2026 · DOIDeveloping more compact and inexpensive platforms that can quantify calcium accurately without auxiliary instrumentation. Investigating the use of the platform in decentralized or resource-limited settings. Exploring the potential of the platform for multiplexing and sensitive readouts.
Rapid Finger-Pump Microfluidic Paper-Based Assay Platform for Monitoring Calcium Ions in Human Biofluids · 2026 · DOIFurther research on the long-term stability of the assays, - Investigation of the assays' performance on other plant pathogens, - Development of more sensitive and specific detection…
Development and Comparison of Visual Colorimetric Endpoint LAMP and Real-Time LAMP-SYBR Green I Assays for Alternaria alternata (Fr.) Keissl in European Plum · 2026 · DOIImproving the dynamic range and sensitivity of the colorimetric readout. Developing automated paper-based valves to reduce user intervention. Exploring applications of the μPAD-based RPA-CRISPR/Cas12a colorimetric platform beyond HPV screening.
Integrated Colorimetric CRISPR/Cas12a Detection of Double-Stranded DNA on Microfluidic Paper-Based Analytical Devices · 2026 · DOIOptimizing thermal cycling uniformity in TV-qPCR, - Improving temperature module stability during high-throughput testing, - Exploring applications of the dual-mode platform in various…
A Portable Dual-Mode Microfluidic Device Integrating RT-qPCR and RT-LAMP for Rapid Nucleic Acid Detection in Point-of-Care Testing · 2026 · DOIDecreased binding efficiency, increased noise, reduced signal-to-noise ratio, and overall lack of reproducibility and reliability in complex clinical samples - Biofouling, non-specific adsorption, and molecular interferences can affect the reliability of the signals - Batch consistency and standardization in compliance with regulations are yet to be…
Point-of-Care Electrochemical Diagnostic Developments for Multidrug-Resistant Bacteria: Role of Aptamers and Nanomaterials · 2026 · DOIExtensive validation and optimization were limited to key reaction parameters, - The presence of at least one sample carrying the minor genotype (MGF = 0.08) was considered sufficient to evaluate assay performance, - The study was limited to a small number of samples…
Rapid Forensic DNA Profiling via Real-Time Recombinase Polymerase Amplification of InDel Markers · 2026 · DOIFurther optimization, such as refined probe design and selection of robust loci, - Integration into portable lab-on-a-chip platforms for rapid, field-deployable forensic…
Rapid Forensic DNA Profiling via Real-Time Recombinase Polymerase Amplification of InDel Markers · 2026 · DOIAddressing the engineering bottlenecks that remain, - Developing more robust correction algorithms for environmental interference and motion…
The storage stability of the electrodes is limited to 3 days, - The electrodes may deteriorate in recognition performance due to the collapse of imprinted sites or deactivation of surface active sites, - Further optimization of storage conditions is needed to extend the shelf life…
Label-Free Impedimetric Biosensor Based on Molecularly Imprinted PPy/MWCNTs Nanocomposites for Sensitive and Selective Detection of Escherichia coli · 2026 · DOIFurther optimization of storage conditions to extend the shelf life of the electrodes, - Investigation of the sensor's performance in more complex samples, - Development of methods to improve the sensor's stability…
Label-Free Impedimetric Biosensor Based on Molecularly Imprinted PPy/MWCNTs Nanocomposites for Sensitive and Selective Detection of Escherichia coli · 2026 · DOIThe study used a limited number of '28 clinical and environmental samples', - The method relies on expensive equipment such as the ABI 7500 real-time quantitative PCR instrument, - The study did not investigate the cost-effectiveness of the MIRA-PfAgo…
Investigating the cost-effectiveness of the MIRA-PfAgo assay, - Exploring the application of the MIRA-PfAgo assay in resource-limited settings, - Developing the MIRA-PfAgo assay for detection of other…
The study has not yet encompassed the full spectrum of clinically relevant concentrations, - The system using the photoresistors showed limited repeatability, - The photodiodes did not demonstrate promising performance, - The necessity for highly specialised or costly equipment may still exist, - The study focused on demonstrating the fundamental feasibility of using such sensors within an integrated analytical…
A Comparison of Cost-Effective Sensors for a Fluorescence-Based Detection System Used in Biodiagnostic Devices · 2026 · DOIFurther enhancements are needed to improve the system's performance, - The full spectrum of clinically relevant concentrations should be encompassed, - Other cost-effective sensors should be investigated, - The system should be tested with different…
A Comparison of Cost-Effective Sensors for a Fluorescence-Based Detection System Used in Biodiagnostic Devices · 2026 · DOI
Most-cited papers in Biosensors and Analytical Detection
- Ultrasensitive and Highly Specific Lateral Flow Assays for Point-of-Care Diagnosis · ACS Nano · 2021 · 620 citations
- Point-of-care diagnostics for infectious diseases: From methods to devices · Nano Today · 2021 · 611 citations
- Wearable materials with embedded synthetic biology sensors for biomolecule detection · Nature Biotechnology · 2021 · 553 citations
- Biosensors applications in medical field: A brief review · Sensors International · 2021 · 492 citations
- Loop-Mediated Isothermal Amplification (LAMP): The Better Sibling of PCR? · Cells · 2021 · 464 citations
- Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives · Sensors · 2021 · 419 citations
- Nanotechnology’s frontier in combatting infectious and inflammatory diseases: prevention and treatment · Signal Transduction and Targeted Therapy · 2024 · 411 citations
- Paper-based microfluidics: Simplified fabrication and assay methods · Sensors and Actuators B Chemical · 2021 · 376 citations
- Colorimetric based on-site sensing strategies for the rapid detection of pesticides in agricultural foods: New horizons, perspectives, and challenges · Coordination Chemistry Reviews · 2021 · 344 citations
- Rapid and ultrasensitive electromechanical detection of ions, biomolecules and SARS-CoV-2 RNA in unamplified samples · Nature Biomedical Engineering · 2022 · 330 citations
Most recent work
- Commercial Translation of Electrochemical Biosensors: Supply Chain Strategy, Scale-Up Manufacturing, and Regulatory–Quality Considerations · Biosensors · 2026
- Machine learning–empowered smartphone platforms for sustainable point-of-care bacterial detection · Talanta · 2026
- Laser-Printed Electrochemical Microfluidic Aptasensing Chip for Simultaneous Detection of Multiple Pesticides · Analytical Chemistry · 2026
- From bench to market: Regulatory and commercial challenges in clinical point-of-care biosensors · TrAC Trends in Analytical Chemistry · 2026
- A handheld fully automated rotating magnetic field-driven integrated tube system with sample-in–answer-out capability · Sensors and Actuators B Chemical · 2026
- Multianalyte nano-biosensor diagnostics: advances through microfluidic and AI integration · Frontiers in Bioengineering and Biotechnology · 2026
- Recent Advances in Nanomaterial-Based Approaches for Plant Disease Management and Early Detection · Journal of Agricultural and Food Chemistry · 2026
- A Review of High-Throughput Optical Sensors for Food Detection Based on Machine Learning · Foods · 2026
- Exploring the Feasibility of Paper-Based Substrates for User-Friendly Electrochemiluminescent Sensors · Analytical Chemistry · 2026
- Nanomaterial-integrated electrogenerated chemiluminescence and resonance energy transfer mechanisms for advanced one-health biosensing · TrAC Trends in Analytical Chemistry · 2026
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