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Biochemistry, Genetics and Molecular Biology · Research topic

Open research questions in Fungal and yeast genetics research

90 unresolved questions extracted from the limitations and future-work sections of 353 Fungal and yeast genetics research papers in our library. Each links back to the study that raised it.

What the literature leaves open

  • further studies on the core catalytic machinery of β-1,6-glucan biosynthesis, - investigation of the regulatory nodes shared with the β-1,3-glucan synthase system, - exploration of the potential applications of β-1,6-glucanases in antifungal therapy

    The Structure, Biosynthesis, and Function of β-1,6-Glucan in the Fungal Cell Wall · 2026 · DOI

  • The core catalytic machinery of β-1,6-glucan biosynthesis remains incompletely defined. The precise biochemical functions of the proteins involved in β-1,6-glucan biosynthesis are unknown. The synthesis processes of β-1,6-glucan vary among different fungal taxa.

    The Structure, Biosynthesis, and Function of β-1,6-Glucan in the Fungal Cell Wall · 2026 · DOI

  • Yet the function of this pathway in this mating-incompetent pathogen remains unclear.

    The transcription factor Ste12(1) from Nakaseomyces glabratus acts pheromone-responsive in S. cerevisiae but seems decoupled from its cognate upstream GPCR in its native host. · 2026 · DOI

  • However, the molecular basis of these interactions remains poorly understood, partly due to challenges in detecting them experimentally.

    Glutathione acts as an exometabolite that promotes growth recovery in fission yeast with defects in amino acid metabolism and cell polarity · 2026 · DOI

  • Abstract Background Successful colonization of the wheat apoplast requires that Zymoseptoria tritici tolerate host-derived stresses, but the mechanisms underlying this adaptation remain poorly understood.

    Contrasting transcriptional responses and genetic determinants underlie Zymoseptoria tritici adaptation mechanisms to simulated host-associated environments · 2026 · DOI

  • The yeast-to-hyphae transition is well understood, but comparatively little is known about the reverse transition from hyphae back to yeast.

    A bacterial metabolite rewires fungal metabolism, triggering the hyphae-to-yeast transition · 2026 · DOI

  • However, its impact on cell wall and membrane homeostasis under ethanol stress remains unclear.

    Nitric oxide enhances ethanol stress tolerance in Wickerhamomyces anomalus through modulating cell membrane and cell wall homeostasis · 2026 · DOI

  • Although CgCFEM1 has been characterized, the role of its closest homolog CgCFEM2 and their potential interplay remains unknown.

    CgCFEM1 and CgCFEM2 modulate virulence in Colletotrichum gloeosporioides by integrated regulation of TOR and cAMP-PKA signaling pathways · 2026 · DOI

  • Most genetic interactions in Saccharomyces cerevisiae have been identified using deletions or overexpression, leaving dosage-dependent interactions underexplored.

    Orthogonal inducible transcriptional control systems for investigation of gene-gene interactions in Saccharomyces cerevisiae · 2026 · DOI

  • Carbon catabolite repression (CCR) and de-repression (CCDR) are critical for fungal development and pathogenicity, yet the underlying regulatory mechanisms remain poorly understood in pathogenic fungi.

    A multilayered regulatory network mediated by protein phosphatase 4 controls carbon catabolite repression and de-repression in Magnaporthe oryzae · 2025 · DOI

  • In vivo functions of the septin and actin cytoskeletons are closely intertwined, yet the mechanisms underlying septin-actin crosstalk have remained poorly understood.

    Sequential recruitment of F-BAR proteins controls cytoskeletal crosstalk at the yeast bud neck · 2025 · DOI

  • 3-methyl pentanoic acid (3MP), a short-chain fatty acid widely employed as a flavoring agent and in agrochemicals, has not been explored for its potential in controlling postharvest diseases or its underlying mechanisms of action.

    3-Methyl pentanoic acid suppress gray mold disease potentially targeting cell-wall integrity (CWI) and mitogen-activated protein kinase (MAPK) pathways in Botrytis cinerea · 2025 · DOI

  • However, comprehensive knowledge of this family in perennial ryegrass remains underexplored.

    Genome-wide analysis of the AP2/ERF transcription factor superfamily in perennial ryegrass · 2025 · DOI

  • However, theories of microbial osmoresponse integrating physical constraints and biological regulations are lacking.

    Physical constraints and biological regulations underlie universal osmoresponses · 2025 · DOI

  • However, it still remains unclear how cells control their cytoplasmic properties in response to environmental cues.

    Cytoplasmic fluidization contributes to breaking spore dormancy in fission yeast · 2024 · DOI

  • Whether closely related non-pathogenic species also exhibit strain heterogeneity remains unknown.

    Strain heterogeneity in a non-pathogenic Aspergillus fungus highlights factors associated with virulence · 2024 · DOI

  • It is possible to increase recombinant protein production by enhancing thermotolerance, but few studies have focused on this topic.

    Coupling thermotolerance and high production of recombinant protein by CYR1N1546K mutation via cAMP signaling cascades · 2024 · DOI

  • Despite its significance, the underlying mechanism of the TOR pathway in Aspergillus flavus remains elusive.

    The target of rapamycin signaling pathway regulates vegetative development, aflatoxin biosynthesis, and pathogenicity in Aspergillus flavus · 2024 · DOI

  • Adaptor protein complex 3 (AP-3) mediates clathrin-independent transport to lysosomes, yet accessory factors supporting this pathway remain incompletely defined.

    Septins associate with AP-3 to support trafficking to the vacuole/lysosome in yeast · 2026 · DOI

  • The biological functions of FocTpc1 in Foc were not well understood. The role of FocTpc1 in growth, reproduction, and pathogenicity was not characterized.

    The Zn2Cys6 transcription factor FocTpc1 is a global regulator of growth, reproduction, pathogenicity, environmental adaptation, and autophagy in Fusarium oxysporum f. sp. cubense · 2026 · DOI

  • The molecular features linking glucose metabolism and redox homeostasis in fungi remain elusive. Understanding the metabolic and redox mechanisms that enable C. chrysosperma to survive and proliferate within host tissues is essential.

    CcAbl1 is required for virulence via regulating NADPH–glutathione-mediated redox balance in Cytospora chrysosperma · 2026 · DOI

  • The role of MoTem1 in M. oryzae was not well understood prior to this study. The study addresses the gap in knowledge by investigating the role of MoTem1 in mitotic regulation and pathogenesis.

    Mutational analysis of the mitotic exit GTPase MoTem1 reveals its role in development, stress adaptation, pathogenicity and global gene regulation in Magnaporthe oryzae · 2026 · DOI

  • Fine mapping is necessary to pinpoint key genes responsible for resistance. Functional mechanisms of the identified QTLs need to be investigated. The development of resistant rice varieties using the identified QTLs is a potential future direction.

    Identification of QTL regions governing neck blast resistance in rice (Oryza sativa L.) · 2026 · DOI

  • The study faced challenges in generating high-confidence models of some killer toxins, such as K74. The study also had to contend with the lack of empirical tertiary structures for Saccharomyces killer toxins.

    A Comprehensive Structural and Functional Analysis of Saccharomyces Killer Toxins · 2026 · DOI

  • further study of the mechanisms of killer toxin carbohydrate binding, oligomerization, and membrane attack, - continued use of the model yeast S. cerevisiae to study killer toxins

    A Comprehensive Structural and Functional Analysis of Saccharomyces Killer Toxins · 2026 · DOI

Most-cited papers in Fungal and yeast genetics research

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90 open questions have been extracted from the limitations and future-work passages of 353 Fungal and yeast genetics research papers in our 4.5M-paper local library. Each one below links back to the study that raised it, so you can read the original claim in context.

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