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Biochemistry, Genetics and Molecular Biology · Research topic

Open research questions in Protein Hydrolysis and Bioactive Peptides

94 unresolved questions extracted from the limitations and future-work sections of 568 Protein Hydrolysis and Bioactive Peptides papers in our library. Each links back to the study that raised it.

What the literature leaves open

  • Investigate the effects of peptides and probiotics in vivo, - Examine the mechanisms of synergy between peptides and probiotics, - Explore the potential applications of peptides and probiotics in food preservation

    Lactiplantibacillus plantarum peptides and probiotics from peanut butter enhance antimicrobial activity and gut microbiota modulation · 2026 · DOI

  • There is a need for further research to translate promising bioactivities into validated health applications. The paper identifies a gap in the understanding of peptide stability, bioaccessibility, gastrointestinal digestion, safety, and biological efficacy.

    Food Proteins and Peptides: Bioactivity, Applications and Health Benefits · 2026 · DOI

  • examining their potential applications in clinical, veterinary, and food-related contexts, - discussing key aspects related to delivery systems, safety, and regulatory considerations, - investigating the use of CDPs in reducing antibiotic use in livestock and humans

    Casein and Casein-Derived Peptides: Antibacterial Activities and Applications in Health and Food Systems · 2025 · DOI

  • Small sample size of three healthy donors, - Limited to in vitro colonic fermentation, - No in vivo experiments were conducted

    Lactiplantibacillus plantarum peptides and probiotics from peanut butter enhance antimicrobial activity and gut microbiota modulation · 2026 · DOI

  • further multidisciplinary research integrating food science, nutrition, protein chemistry, and microbiology

    Food Proteins and Peptides: Bioactivity, Applications and Health Benefits · 2026 · DOI

  • However, current evaluation approaches, such as two-dimensional cell cultures and animal models, remain insufficient to capture the complex interactions underlying peptide-driven immune regulation.

    Marine-Derived Bioactive Peptides as Immunomodulatory Agents: Organoid-Based Platforms and Translational Perspectives · 2026 · DOI

  • Nonetheless, there is limited literature addressing real-life applications in veterinary medicine, food safety, and public health.

    Casein and Casein-Derived Peptides: Antibacterial Activities and Applications in Health and Food Systems · 2025 · DOI

  • However, no studies have reported on the production of antioxidant peptides from hijiki proteins.

    Antioxidant Peptides from Hizikia fusiformis: A Study of the Preparation, Identification, Molecular Docking, and Cytoprotective Function of H2O2-Damaged A549 Cells by Regulating the Keap1/Nrf2 Pathway · 2025 · DOI

  • Recently, increasing evidence has revealed the excellent wound-healing activity of collagen peptides, but their molecular mechanisms remain incompletely elucidated.

    Deciphering the Wound-Healing Potential of Collagen Peptides and the Molecular Mechanisms: A Review · 2024 · DOI

  • The challenges and future application perspectives of these bioactive peptides also need to be explored.

    Nutrition and Cardiovascular Disease: The Potential Role of Marine Bioactive Proteins and Peptides in Thrombosis Prevention · 2024 · DOI

  • Rice protein peptide (RPP) has been reported to alleviate the symptoms of dextran sulfate sodium (DSS)-induced colitis, but its potential protective effect and fundamental neurobiological mechanisms against DSS-induced inflammatory bowel disease (IBD), coupled with depression and cognitive impairment, remain unclear.

    Rice Protein Peptides Ameliorate DSS-Induced Cognitive Impairment and Depressive Behavior in Mice by Modulating Phenylalanine Metabolism and the BDNF/TRKB/CREB Pathway · 2024 · DOI

  • Further investigation of the ZO-1-mediated interaction between NCW and tight junction proteins will contribute to a better understanding of the paracellular transport mechanism of NCW.

    Understanding the Transepithelial Transport and Transbilayer Diffusion of the Antihypertensive Peptide Asn-Cys-Trp: Insights from Caco-2 Cell Monolayers and the DPPC Model Membrane · 2024 · DOI

  • However, the underlying mechanisms of what peptides bind and how they bind iron ions remain unclear.

    Hemoglobin Hydrolyzate Promotes Iron Absorption in the Small Intestine through Iron Binding Peptides · 2024 · DOI

  • Beyond the physiological effects of peptides, their bioavailability to the brain still remains unclear since the blood-brain barrier (BBB) strictly controls the transportation of compounds to the brain.

    Impact of Peptide Transport and Memory Function in the Brain · 2024 · DOI

  • The interplays between bioactive peptides and gut microbiota are not fully understood, but bioactive peptides hold promise as modulators of the gut microbiota to promote gut health.

    Effect of Bioactive Peptides on Gut Microbiota and Their Relations to Human Health · 2024 · DOI

  • However, little is known about the fermentation processes of cereals with these starters.

    Fermentation of Rice, Oat, and Wheat Flour by Pure Cultures of Common Starter Lactic Acid Bacteria: Growth Dynamics, Sensory Evaluation, and Functional Properties · 2024 · DOI

  • While multi-product cascade biorefining schemes show promise due to their greater techno-economic viability, several biotechnological hurdles remain to be overcome at many levels.

    Food Waste Biotransformation into Food Ingredients: A Brief Overview of Challenges and Opportunities · 2024 · DOI

  • However, few studies have focused on edible Pleurotus eryngii mushroom feet peptides (PEMFPeps).

    Identification of Peptides from Edible Pleurotus eryngii Mushroom Feet and the Effect of Delaying D-Galactose-Induced Senescence of PC12 Cells Through TLR4/NF-κB/MAPK Signaling Pathways · 2024 · DOI

  • Thus, waste from the shrimp industry in conjugation with plant polyphenols could be utilized to produce antioxidant and antidiabetic or anti-obesity agents, which could be explored as a promising additive in functional foods and nutraceuticals.

    Enhanced Antioxidant and Digestive Enzyme Inhibitory Activities of Pacific White Shrimp Shell Protein Hydrolysates via Conjugation with Polyphenol: Characterization and Application in Surimi Gel · 2024 · DOI

  • The present study revealed the presence and activity of DPP-IV inhibitory peptides in goat milk, providing important insights for further investigation of their potential food functionality and health benefits.

    DPP-IV Inhibitory Peptide against In Vitro Gastrointestinal Digestion Derived from Goat’s Milk Protein and Its Activity Enhancement via Amino Acid Substitution · 2024 · DOI

  • The study can be extended to include specific pathogens of interest for food packaging applications. The newly generated multifunctional bioactive peptides can be validated experimentally.

    Design of Multifunctional Bioactive Peptides for Potential Application in Food Packaging: A Machine-Learning-Integrated Optimization Framework · 2026 · DOI

  • There is a need for novel food packaging materials that can extend the shelf life of food. There is a need for machine-learning-based approaches for designing multifunctional bioactive peptides.

    Design of Multifunctional Bioactive Peptides for Potential Application in Food Packaging: A Machine-Learning-Integrated Optimization Framework · 2026 · DOI

  • There is a need to investigate the protective effects of whey protein concentrate against intestinal toxicity. The mechanisms of antioxidant peptides in reducing oxidative stress and inflammation are not fully understood. The potential applications of whey protein concentrate as a nutritional treatment for intestinal disorders require further research.

    In silico molecular docking and in vivo evidence of whey protein concentrate-mediated protection against thioacetamide-induced intestinal toxicity in male albino rats via modulation of oxidative stress, inflammation, apoptosis, and fibrosis · 2026 · DOI

  • To validate the docking scores using redocking or RMSD analysis. To perform ADMET prediction to assess the biological relevance and translational applicability of the results. To conduct experimental assays to strengthen the reliability and biological importance of the results.

    Computational Identification of GPCR-Targeting Bioactive Compounds from Ageratum conyzoides for Anti-Aging · 2026 · DOI

  • There is a need to identify bioactive compounds that target GPCRs associated with aging mechanisms. There is a lack of studies using computational methods to investigate the binding affinities of these compounds.

    Computational Identification of GPCR-Targeting Bioactive Compounds from Ageratum conyzoides for Anti-Aging · 2026 · DOI

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94 open questions have been extracted from the limitations and future-work passages of 568 Protein Hydrolysis and Bioactive Peptides papers in our 4.5M-paper local library. Each one below links back to the study that raised it, so you can read the original claim in context.

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