Open research questions in Wheat and Barley Genetics and Pathology
85 unresolved questions extracted from the limitations and future-work sections of 521 Wheat and Barley Genetics and Pathology papers in our library. Each links back to the study that raised it.
What the literature leaves open
The study identifies a gap in understanding the effects of genotype, location, and growing season on grain yield and quality traits in winter wheat genotypes.
Evaluation of DUS and VCU Traits in Winter Wheat (Triticum aestivum L.) Genotypes Across Multiple Locations and Seasons · 2026 · DOIThe study identifies a gap in the understanding of genetic resistance mechanisms for wheat production. The study aims to address this gap by evaluating the resistance levels of wheat lines to leaf and stripe rust under natural conditions.
Mapping resistance to leaf and stripe rusts in bread Triticum aestivum L. germplasm through GWAS and field phenotyping · 2026 · DOIFurther study is needed to investigate the effects of the translocation in multiple genetic backgrounds. The potential pleiotropic effects of the translocation on other traits should be investigated. The study of other genetic variations that affect culm length could provide new insights into the genetic regulation of this trait.
A translocation-mediated duplication of the distal region of chromosome 5A reduced the culm length in a gamma-irradiated wheat mutant · 2026 · DOIThere is a need for novel genetic variations that can be used to fine-tune culm length in wheat. Current semi-dwarf genes have pleiotropic effects and are not ideal for use in breeding programs.
A translocation-mediated duplication of the distal region of chromosome 5A reduced the culm length in a gamma-irradiated wheat mutant · 2026 · DOIThere is a need for further investigations into the effects of epimutagens on crop variability. The long-term stability of epimutagen-induced traits is unknown.
Wheat yellow rust is a significant problem in the Ethiopian highlands. The disease reduces production and affects seed or grain quality. There is a need to develop effective control strategies.
Association of biophysical and agronomic factors influencing yellow rust (Puccinia striformis f.sp. tritici) epidemic on bread wheat in the Southern Highlands of Ethiopia · 2026 · DOIStemphylium blight is a serious disease affecting lentil crops. The disease causes significant yield losses. There is a gap between the expected yield and the actual yield of lentil crops.
Genetic diversity analysis among lentil genotypes for stemphylium blight tolerance using SSR markers · 2026 · DOILodging resistance and grain yield are affected by plant height. Rht5 influences seedling vigor and coleoptile length. The molecular mechanism of Rht5-mediated plant height regulatory pathway is unclear.
Further studies are needed to elucidate the molecular mechanism of Rht5-mediated plant height regulatory pathway. The functional role of TaGAD2 in regulating plant height needs to be further investigated.
The Lo7 genome assembly is near-complete, but not T2T. The assembly may still contain errors or gaps.
Unveiling centromeric retrotransposon dynamics through a high-quality rye genome assembly · 2026 · DOIIntegrating more diverse genomic data sources, such as transcriptomics. Performing more comprehensive gene family analyzes. Applying the integration of multiple sequencing technologies to other complex genomes.
Unveiling centromeric retrotransposon dynamics through a high-quality rye genome assembly · 2026 · DOIThe conversion of valuable agricultural land to urbanization. The need to enhance wheat production to meet the food demand of the growing population.
General and specific combining ability analysis in lines, testers, and hybrids (F1/F2) of Triticum aestivum L. · 2026 · DOIGrain color is closely associated with tannin content, yet the genetic mechanism underlying grain color formation remains incompletely understood.
Identification and Fine Mapping of a MYB-like Transcription Factor Regulating Yellow Grain in Sorghum bicolor · 2026 · DOIHowever, the physiological characteristics and molecular basis underlying yield increases in intercropped wheat remain insufficiently understood.
Comprehensive analysis of physiological characteristic and global transcriptional profiling to unravel the mechanism of wheat yield increase in intercropping · 2026 · DOIRainfed agriculture is critically dependent on seasonal precipitation, yet the relationship between rainfall amount, its temporal distribution, and crop yield remains poorly understood—particularly in water-limited, Mediterranean-type environments.
While stem biomass investment is known to promote grain-filling under stress, its underlying genetic architecture remains poorly understood.
Antagonistic pleiotropy at the stem solidness 1 locus balances wheat stem architecture and yield components under terminal drought · 2026 · DOIBMC Plant Biology (2026) 26:750 environments with their training-set means, reflecting a realistic breeding situation in which future environmen- tal conditions are not yet known at selection time.
Multimodal deep learning improves cross-environment prediction of durum wheat yield components · 2026 · DOIAlthough the paper confirms significant G×PS interactions for protein and gluten content (P < 0.001), it does not investigate the temporal dynamics of protein and gluten accumulation during grain fill, or whether these differences result from altered translocation patterns, differential remobilization from vegetative tissues, or enhanced de novo synthesis under high-intensive conditions.
Performance of Spring Wheat Genotypes for Quality, Yield, and Related Reproductive Traits under Different Production Systems · 2026 · DOIThe study demonstrates that Belyana achieves maximum grain yield (5.06 t ha⁻¹) under high-intensive production, but provides no analysis of the specific agronomic input levels (nitrogen rates, irrigation amounts, pest management intensity) driving this performance. Quantified input-response curves for each genotype-production system combination are needed to establish threshold input levels and diminishing return points.
Performance of Spring Wheat Genotypes for Quality, Yield, and Related Reproductive Traits under Different Production Systems · 2026 · DOI1. The experiment was conducted only at Oke-Oyi, which may limit the generalisation of results across diverse agro-ecological zones in SSA. 83 Page 12 of 13 Plant Molecular Biology Reporter (2026) 44:83 2. Although conducted over two cropping seasons, this duration may not fully capture long-term environmental variability and genotype × environment interactions. 3. The use of only two replications may reduce the preci- sion of variance estimates and affect the robustness of statistical inference. 4. The study evaluated 16 inbred lines and two checks, which may not fully represent the broader genetic diver- sity available in PVA-QPM germplasm. 5. The molecular analysis relied on a limited set of mark- ers (SCAR, SNP, and KASP linked to GLS loci), which may not capture the full genomic architecture underlying GLS resistance and nutritional traits.
Marker-Assisted Selection for Gray Leaf Spot Resistance in Extra-Early Provitamin A Quality Protein Maize Inbred Lines · 2026 · DOIInvestigating the distinct parts of the oat panicle in relation to endogenous hormones remains a research gap for understanding the physiological mechanisms underlying yield formation.
The role of endogenous hormones in regulating grain number in oat panicles under drought stress across cultivars · 2026 · DOIThe study identifies a gap in our understanding of the long-term virulence dynamics of Bgt and the durability of resistance genes. The study highlights the need for a comprehensive analysis of Bgt populations over time to inform resistance management strategies.
Deciphering a pathogen’s evolution: a two-decade longitudinal study reveals virulence shifts and identifies durable Pm genes against Himalayan Blumeria graminis f. sp. tritici populations · 2026 · DOIThe lack of drought-tolerant wheat cultivars threatens global food security. There is a need to evaluate drought tolerance in diverse hexaploid wheat accessions. The genetic diversity and drought adaptive potential of hexaploid wheat accessions are not well understood.
Assessing Genetic Diversity and Drought Adaptive Potential in Diverse Hexaploid Wheat Accessions · 2026 · DOITraditional visual disease severity assessments often fail to accurately predict grain yield. There is a need for more reliable and accurate methods for predicting grain yield.
Healthy area of the penultimate leaf (F2) as a predictor of barley grain yield under foliar disease pressure in semi-arid environments · 2026 · DOIHeat stress is a major constraint to global crop productivity, particularly in wheat. Stomata play a central role in balancing photosynthetic carbon assimilation and evaporative cooling, but the mechanisms underlying this process are not well understood. Breeding programs aimed at improving wheat heat tolerance are limited by a lack of understanding of the genetic architecture underlying stomatal traits.
QTLs for heat-induced stomatal anatomy underpin gas exchange variation in field-grown wheat · 2026 · DOI
Most-cited papers in Wheat and Barley Genetics and Pathology
- Pan-genome bridges wheat structural variations with habitat and breeding · Nature · 2024 · 148 citations
- Harnessing landrace diversity empowers wheat breeding · Nature · 2024 · 142 citations
- Structural variation in the pangenome of wild and domesticated barley · Nature · 2024 · 131 citations
- Origin and evolution of the bread wheat D genome · Nature · 2024 · 86 citations
- Production vulnerability to wheat blast disease under climate change · Nature Climate Change · 2024 · 84 citations
- Effects of salinity stress on seedling biomass, physiochemical properties, and grain yield in different breeding wheat genotypes · Acta Physiologiae Plantarum · 2021 · 44 citations
- Different proline responses of two Algerian durum wheat cultivars to in vitro salt stress · Acta Physiologiae Plantarum · 2020 · 36 citations
- The origin of the B-genome of bread wheat (Triticum aestivum L.) · Russian Journal of Genetics · 2013 · 28 citations
- The role of genetic diversity and pre‐breeding traits to improve drought and heat tolerance of bread wheat at the reproductive stage · Food and Energy Security · 2023 · 22 citations
- Unlocking Wheat Drought Tolerance: The Synergy of Omics Data and Computational Intelligence · Food and Energy Security · 2024 · 16 citations
Most recent work
- The Complex Landscape of Multidrug Resistance in Zymoseptoria tritici: MFS1 Promoter Plasticity and beyond · bioRxiv · 2026
- The potential of considering photosynthesis parameters in crop yield breeding by genomic prediction · Journal of Experimental Botany · 2026
- Functional 5Bq allele is responsible for the compact spike phenotype in common wheat (Triticum aestivum L.) · BMC Plant Biology · 2026
- Multimodal deep learning improves cross-environment prediction of durum wheat yield components · BMC Plant Biology · 2026
- Evaluation of morpho-biological indicators of the winter wheat RIL population resistant to yellow rust · Plant Science Today · 2026
- Performance of Spring Wheat Genotypes for Quality, Yield, and Related Reproductive Traits under Different Production Systems · Advances in Biology & Earth Sciences · 2026
- Field scale evaluation of agronomic and malting quality traits in hybrid barley · BMC Plant Biology · 2026
- A systems biology and QTL-based dissection of germination traits in Iranian wheat under normal and water-limited environments · Scientific Reports · 2026
- Biological Properties of Spelt Wheat and Prospects of Its Use in Breeding · Agricultural Science and Practice · 2026
- Development and molecular cytogenetic identification of a new wheat-Psathyrostachys huashanica Keng translocation line with resistances to stripe rust · BMC Plant Biology · 2026
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