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Open research questions in Genetic Mapping and Diversity in Plants and Animals

71 unresolved questions extracted from the limitations and future-work sections of 261 Genetic Mapping and Diversity in Plants and Animals papers in our library. Each links back to the study that raised it.

What the literature leaves open

  • Fine mapping and gene cloning of the qDEC7.01 QTL. Development of rice varieties with reduced chalkiness using marker-assisted selection.

    Conjunctive analysis of BSA-Seq and KASP markers unveils the preliminary candidate genes controlling chalkiness of rice grain · 2026 · DOI

  • The genetic mechanisms underlying grain chalkiness remain to be fully elucidated. There is a need to identify the genetic basis of grain chalkiness and offer potential targets for improving rice quality.

    Conjunctive analysis of BSA-Seq and KASP markers unveils the preliminary candidate genes controlling chalkiness of rice grain · 2026 · DOI

  • The complexity of the trait, powdery mildew resistance, makes it challenging to develop accurate predictive models. The limited population size and marker density may affect the accuracy of the results. The need to consider trait genetic architecture to enhance predictive abilities is a challenge.

    Genomic prediction of wild-derived powdery mildew resistance for strawberry (Fragaria × ananassa) pre-breeding · 2026 · DOI

  • The complex regulation of starch biosynthesis in rice. The limited understanding of the wx allele's role in grain quality and yield traits. The need for efficient and precise gene editing tools.

    Multifaceted Effects of Starch Branching Enzyme and Soluble Starch Synthase Gene Editing in Rice with Different Wx Genotypes · 2026 · DOI

  • Lodging susceptibility is influenced by multiple factors, including plant height, culm diameter, and nitrogen application. The current reliance on the sd1 gene has proven insufficient for ensuring effective lodging resistance. There is a need for integrated strategies to mitigate lodging under variable environments.

    Recent physiological, genetic, and management perspectives on lodging resistance in rice · 2026 · DOI

  • The study only used 19 cowpea accessions, which may not be representative of the entire cowpea population. The KASP markers were only tested for their ability to discriminate the 19 accessions, and their performance on other accessions is unknown.

    Development of KASP molecular markers and construction of fingerprinting for cowpea (Vigna unguiculata (L.) Walp.) based on ddRAD-Seq · 2026 · DOI

  • Further research is needed to test the performance of the KASP markers on other cowpea accessions. The development of more KASP markers for cowpea could improve the efficiency of breeding programs. The use of other molecular markers, such as SSRs or SNPs, could be explored for cowpea breeding.

    Development of KASP molecular markers and construction of fingerprinting for cowpea (Vigna unguiculata (L.) Walp.) based on ddRAD-Seq · 2026 · DOI

  • The lack of a reference genome for radish has hindered the understanding of the genetic basis of agronomic traits. The genetic basis of high cellulose accumulation in radish remains largely unexplored.

    Chromosome-level genome assembly of landrace ‘Huangzhou’ provides insights into high cellulose accumulation in radish · 2026 · DOI

  • Despite its importance, the genetic and molecular mechanisms governing carrot VR remain elusive.

    A pair of duplicated SOC1 genes, DcSOC1-1 and DcSOC1-2, control vernalization requirement in carrot · 2026 · DOI

  • While functional links between stomatal and vascular traits are known in dicots, their potential genetic coordination in C4 crops remains poorly understood.

    AI-enabled simultaneous phenotyping of leaf vein and stomatal traits uncovers independent genetic control in maize · 2026 · DOI

  • Limited information exists regarding cis-acting regulatory elements and candidate genes controlling the heat shock transcription factor (HSF) gene family in rice.

    Genome-Wide Characterization and Expression Analysis of Heat Shock Transcription Factors in Two Cultivars of Rice (Oryza sativa L.) Under Heat Stress · 2026 · DOI

  • The review identifies persistent knowledge gaps—including the limited translation of genomic advances to smallholder contexts and the underexplored potential of microbiome engineering—and calls for a convergence of disciplinary expertise, equitable technology transfer, and coherent policy support to achieve drought-resilient food systems globally.

    Drought-Resilient Crops for Sustainable Agriculture: Genetic, Physiological, and Agronomic Advances · 2026 · DOI

  • However, the genetic basis underlying chlorophyll degradation and its environmental responsiveness remains incompletely understood.

    Identification and Fine Mapping of qCD2, a Major QTL Governing Leaf Premature Senescence in Rice (Oryza sativa L.) · 2026 · DOI

  • This study provides a cultivar-specific genomic resource for PG56 that can support further investigation of the genetic basis of the submergence escape response.

    De novo genome assembly of the deepwater rice (Oryza sativa) cultivar Pin Gaew 56 · 2026 · DOI

  • Thermo-sensitive genic male sterile lines in neo-tetraploid rice have demonstrated hybrid vigor through gene editing, but scaling these breeding approaches to practical deployment at the farm level with climate variability projections remains underdeveloped. Integration of climate-adaptive breeding strategies with speed breeding protocols and phenotypic selection across transient heat stress periods during early grain development needs validation.

    Harnessing climate-adaptive breeding to accelerate tolerance to extreme temperatures in rice · 2026 · DOI

  • Natural variation in cold tolerance genes (CTF1) and high-temperature tolerance genes (HTH5) from wild rice species Oryza rufipogon has been documented, but systematic screening and introgression of these alleles into elite breeding lines using genome-wide association studies and genomic selection remains incomplete. Precision characterization of gene-by-environment interactions for temperature tolerance across diverse germplasm collections is needed.

    Harnessing climate-adaptive breeding to accelerate tolerance to extreme temperatures in rice · 2026 · DOI

  • With the development of these components, AI-driven GP will transition from a theoretical framework to the foundational basis for predictive breeding decisions. These systems will speed up genetic gain, and help farming stay viable in a changing climate.

    Beyond QTL and GWAS: how deep learning, graph models, and multi-omics are reshaping plant genomic prediction analysis · 2026 · DOI

  • Further study of the genetic loci associated with mesocotyl length. Use of the identified genetic loci in breeding programs. Investigation of other traits associated with seedling emergence and establishment.

    Identification of Genetic Loci for Mesocotyl Length in Rice (Oryza sativa L.) using Genome-Wide Association Approach · 2026 · DOI

  • Studies on cultivar adaptation to a changing Mediterranean climate - Research on high-value cultivars rising in importance - Further analysis of gene regulatory networks and genetic variability

    Integrative Genomics and Phenotyping Strategies to Advance Sustainable Cultivation of European Rice Varieties with Enhanced Nutritional Quality · 2026 · DOI

  • The need to understand how rice interacts with its environment and how different varieties have adapted to diverse climates - The need to develop nutritionally enhanced rice varieties

    Integrative Genomics and Phenotyping Strategies to Advance Sustainable Cultivation of European Rice Varieties with Enhanced Nutritional Quality · 2026 · DOI

  • Limited haploid seed availability prevented advancement of some genotypes to Screening Stage 2. The study relied on a specific dataset of 295 haploid lines.

    A two-stage screening approach integrated with GWAS for rare phenotypes such as spontaneous haploid genome doubling in maize · 2026 · DOI

  • Further evaluation of the two-stage SHGD screening approach in different environments. Investigation of the genetic mechanisms underlying SHGD. Development of new DH breeding strategies using the identified genotypes with stable SHGD potential.

    A two-stage screening approach integrated with GWAS for rare phenotypes such as spontaneous haploid genome doubling in maize · 2026 · DOI

  • Further studies can investigate the genetic basis of NCLB resistance in other maize populations. The development of tailored breeding strategies for durable resistance can be explored.

    Genetic Insights into NCLB Resistance in Maize (Zea Mays L.) Populations Derived from NEHR Landraces Using Generation Mean Analysis · 2026 · DOI

  • There is a need to understand the underlying gene action governing NCLB resistance. Prior work has focused on qualitative resistance, which is often short-lived.

    Genetic Insights into NCLB Resistance in Maize (Zea Mays L.) Populations Derived from NEHR Landraces Using Generation Mean Analysis · 2026 · DOI

  • The absence of a Reference genome for kodo millet hampers the comprehensive identification of genes associated with abiotic stress responses. A complete draft genome for barnyard millet remains unavailable, with current data limited to partial genomic resources from barnyard grass. The molecular mechanisms of abiotic stress responses in kodo millet and barnyard millet are not well understood.

    Harnessing genomics and transcriptomics to explore the genetic and molecular basis of abiotic stress tolerance in minor millets – a comprehensive review · 2026 · DOI

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71 open questions have been extracted from the limitations and future-work passages of 261 Genetic Mapping and Diversity in Plants and Animals papers in our library. Each one below links back to the study that raised it, so you can read the original claim in context.

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