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Open research questions in Identification and Quantification in Food

127 gap statements mined from Identification and Quantification in Food papers in our 4.5M-paper local library, which holds 684 papers on the topic — drawn mostly from each paper's own stated research gap, future-work, challenge and limitation notes, and its abstract. The ones listed below are a selection still marked open; each names the study that raised it, with a DOI link where the paper has one.

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What the literature leaves open

  • Rapid and cost-effective tools for species authentication of closely related Euterpe taxa in processed commercial matrices remain limited. Current molecular methods have limitations, such as amplification failure and mixed chromatograms.

    High-resolution melting analysis of chloroplast markers for species authentication and mislabeling detection in commercial açaí and juçara products · 2026 · DOI

  • Current methods for species identification may not be effective in preventing commercial fraud and protecting consumer health. There is a need for a reliable and efficient method for species identification of crustaceans.

    Species identification of shrimps, lobsters and crayfish using peptide-based liquid chromatography low-resolution mass spectrometry and random forest model · 2026 · DOI

  • Global advancement is nonetheless hampered by disparities in testing criteria. There is a lack of consensus across testing centers. There is disproportionate accessibility to technology across nations.

    Integration of advanced analytical techniques, chemometrics, artificial intelligence, and regulatory approaches for food fraud detection and global food integrity · 2026 · DOI

  • The lack of effective methods for detecting fish gelatin in commercial products due to DNA degradation during gelatin production. The need for a reliable and efficient method for verifying the source of gelatin in commercial products.

    Comparative performance of DNA and peptide-based methods for fish gelatin detection in commercial products · 2026 · DOI

  • The need for a reliable and efficient method for food authentication, particularly for halal authentication. The lack of a comprehensive workflow integrating rapid screening and molecular confirmation methods.

    Chemometric-guided ATR-FTIR screening and GC–MS confirmation for authentication of lard and beef tallow: a halal-oriented analytical workflow · 2026 · DOI

  • There is a need to investigate the effects of edible bird's nest and free sialic acids on LPS-induced brain inflammation in mice. The mechanisms of edible bird's nest and free sialic acids on brain inflammation are not well understood.

    Interventional Effects of Edible Bird’s Nest and Free Sialic Acids on LPS-Induced Brain Inflammation in Mice · 2025 · DOI

  • There is a lack of holistic reviews that comprehensively integrate and evaluate technological developments in animal identification. Prior efforts have focused on specific methods or time periods, without a comprehensive overview. The paper aims to fill this gap by providing a historical perspective on animal identification methods.

    Advancements in Individual Animal Identification: A Historical Perspective from Prehistoric Times to the Present · 2025 · DOI

  • One challenge is the limitations of traditional detection methods in detecting food adulteration. Another challenge is the need for more robust regulatory frameworks and food safety policies. The susceptibility of NGS to primer bias is also a challenge.

    Current Progress and Future Trends of Genomics-Based Techniques for Food Adulteration Identification · 2025 · DOI

  • The reliance on reference databases is a key limitation in yeast identification.

    Detection and Identification of Food-Borne Yeasts: An Overview of the Relevant Methods and Their Evolution · 2025 · DOI

  • There is a lack of research on antimicrobial resistance in wild birds, particularly in threatened species. The study aims to fill this gap by exploring the prevalence of antimicrobial resistance in Bonelli's eagles. The research gap includes the need to understand the mechanisms of resistance acquisition and transmission.

    Exploring the Prevalence of Antimicrobial Resistance in the Environment Through Bonelli’s Eagles (Aquila fasciata) as Sentinels · 2025 · DOI

  • The diet of the Malayan Krait is largely unknown. The species' trophic interactions are not well understood. Prior work has not considered fish as potential prey for Malayan Kraits.

    A Documented Case of Ichthyophagy in the Malayan Krait (Bungarus candidus) and Its Implications for Toxinology · 2025 · DOI

  • Traditional satellite data are limited in fine-scale mangrove species classification due to low spatial resolution and less spectral information. Sole reliance on spectral reflectance for mangrove species classification has limitations. The study does not mention any specific limitations of the methodology used.

    Fine-Scale Mangrove Species Classification Based on UAV Multispectral and Hyperspectral Remote Sensing Using Machine Learning · 2024 · DOI

  • Further research could explore the application of UAV hyperspectral images using machine learning models for other ecological management and conservation strategies. The use of other machine learning models or feature extraction techniques could be investigated. The study of mangrove species classification using UAV hyperspectral images could be extended to other regions or environments.

    Fine-Scale Mangrove Species Classification Based on UAV Multispectral and Hyperspectral Remote Sensing Using Machine Learning · 2024 · DOI

  • There is a lack of studies on species misrepresentation in cat food products. Prior work on molecular authentication of cat food products is limited. There is a need for a dependable barcoding reference sequence database to verify cases of mislabeling.

    DNA mini-barcoding reveals the mislabeling rate of canned cat food in Taiwan · 2024 · DOI

  • The mitochondrial evolution and diversity of Schistura are still not well understood. Insufficient morphological and genetic data for most Schistura species have hindered the ability to accurately diagnose species groups as genera.

    Mitochondrial Genome Diversity of Schistura McClelland, 1838 (Teleostei, Nemacheilidae) · 2024 · DOI

  • The need to distinguish between naturally recruited and introduced eels in the Baltic Lakeland region.

    Genetic Diversity and Distribution of Haplotypes of Freshwater Eel in Baltic Lakeland Based on Mitochondrial DNA D-Loop and Cytochrome b Sequence Variation · 2024 · DOI

  • Further studies could investigate the application of HRM analysis to other commercial products, - Research could focus on improving the detection limit of HRM analysis, - Studies could explore the use of HRM analysis in conjunction with other methods for species…

    High-resolution melting analysis of chloroplast markers for species authentication and mislabeling detection in commercial açaí and juçara products · 2026 · DOI

  • The significant natural variability of food commodities - The insertion on the market of products that have passed many times through a subtle and hardto-detect adulteration process - The need for laborious, highly sensitive analytical…

    The Journey of Artificial Intelligence in Food Authentication: From Label Attribute to Fraud Detection · 2025 · DOI

  • The resolution of 16S rRNA gene amplicon sequencing is limited, - It is susceptible to primer bias, - qPCR relies on Cq analysis, - The widespread issue of adulteration in the dairy industry has created a demand for reliable detection…

    Current Progress and Future Trends of Genomics-Based Techniques for Food Adulteration Identification · 2025 · DOI

  • Investigating the application of FreshFusionNet in other food products, - Improving the robustness of the model to various environmental factors, - Exploring the use of other types of indicator…

    A Smartphone-Based Non-Destructive Multimodal Deep Learning Approach Using pH-Sensitive Pitaya Peel Films for Real-Time Fish Freshness Detection · 2025 · DOI

  • Limited attention has been given to plant-based proteins in food fraud prevention, - Most efforts have focused on animal-based products, - The review only covers publications from 2010…

    Food Fraud in Plant-Based Proteins: Analytical Strategies and Regulatory Perspectives · 2025 · DOI

  • Prospective insights and future research directions are needed to reinforce integrity and transparency within the plant-based protein market, - Further studies on advanced analytical approaches for detecting plant-based protein fraud are required, - Research on the economic implications of food fraud in plant-based proteins…

    Food Fraud in Plant-Based Proteins: Analytical Strategies and Regulatory Perspectives · 2025 · DOI

  • Small sample size of 10 individuals, - Limited geographic scope to southeast-south Brazilian waters, - No explicit statement…

    A Crusade Throughout the World’s Oceans: Genetic Evidence of the Southern Bluefin Tuna Thunnus maccoyii and the Pacific Bluefin Tuna Thunnus orientalis in Brazilian Waters · 2025 · DOI

  • Further study of the migration patterns of T. orientalis and T.

    A Crusade Throughout the World’s Oceans: Genetic Evidence of the Southern Bluefin Tuna Thunnus maccoyii and the Pacific Bluefin Tuna Thunnus orientalis in Brazilian Waters · 2025 · DOI

  • There needs to be more studies and method development before the camera-based identification system employed…

    Advancements in Individual Animal Identification: A Historical Perspective from Prehistoric Times to the Present · 2025 · DOI

Most-cited papers in Identification and Quantification in Food

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Open questions raised by papers listed on this page include: (1) Rapid and cost-effective tools for species authentication of closely related Euterpe taxa in processed commercial matrices remain limited (“High-resolution melting analysis of chloroplast markers for species…”, 2026); (2) There is a need for a reliable and efficient method for species identification of crustaceans (“Species identification of shrimps, lobsters and crayfish using peptide-based…”, 2026); (3) The lack of effective methods for detecting fish gelatin in commercial products due to DNA degradation during gelatin production (“Comparative performance of DNA and peptide-based methods for fish gelatin…”, 2026); (4) The lack of a comprehensive workflow integrating rapid screening and molecular confirmation methods (“Chemometric-guided ATR-FTIR screening and GC–MS confirmation for authentication…”, 2026); (5) There is a need to investigate the effects of edible bird's nest and free sialic acids on LPS-induced brain inflammation in mice (“Interventional Effects of Edible Bird’s Nest and Free Sialic Acids on…”, 2025). 127 gap statements have been mined from Identification and Quantification in Food papers in our 4.5M-paper local library, which holds 684 papers on the topic; the gaps come from whichever of those papers state one. They are mostly the research gaps the authors state and the papers' abstracts, plus future-work, limitations and challenges passages. The ones listed below are a selection still marked open; each names the study that raised it, with a DOI link where the paper has one, so you can read the original claim in context.

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