Open research questions in Moringa oleifera research and applications
184 gap statements mined from Moringa oleifera research and applications papers in our 7.9M-paper local library, which holds 513 papers on the topic — drawn mostly from each paper's own stated research gap, future-work, challenge and limitation notes, and its abstract. The ones listed below are a selection still marked open; each names the study that raised it, with a DOI link where the paper has one.
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What the literature leaves open
Despite robust preclinical and preliminary clinical evidence supporting the antihypertensive and endothelium-protective properties of M. oleifera, several critical research gaps must be addressed before its integration into clinical practice. A significant portion of the current literature relies on animal models or small-scale, short-term human trials.
Moringa oleifera for blood pressure regulation and endothelial dysfunction – Mechanisms and therapeutic potential in cardiovascular disease: A review · 2026 · DOIFuture research should focus on monitoring enzymatic activity during SSF, as well as on developing metabolomic profiles to track phenolic transformations and assessing safety-related aspects, including the potential production of mycotoxins.
Fungal-Assisted Extraction-SSF of Phenolic Compounds from Moringa oleifera and Its Effects on Antimicrobial and Antioxidant Properties · 2026 · DOIThese improvements suggest that MOLE supplementation has the potential for enhanced fertility in native Thai bulls, warranting further investigation through in vivo studies to assess pregnancy rates and reproductive efficiency.
Enhancement of Semen Cryopreservation from Native Thai Bulls Through Moringa oleifera Leaf Extract Supplementation · 2025 · DOIFuture directions for its use in this context should focus on expanding research and clinical applications. While preclinical studies suggest MO’s potential in treating various CDs, large-scale clinical trials are essential to validate its safety, efficacy, and dosage across diverse populations.
Unveiling the Miracle Tree: Therapeutic Potential of Moringa oleifera in Chronic Disease Management and Beyond · 2025 · DOIFurther studies should be conducted to confirm whether tannin, phytic acid, and saponin are responsible for the insignificant growth performance of Nile tilapia. Further research is warranted to elucidate the molecular mechanisms underlying the effects of MLE on Nile tilapia following bacterial infection.
Effect of Moringa oleifera Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae Biotype 2 · 2024 · DOIThere is a need for alternative feed supplements to improve disease resistance in Nile tilapia. The use of natural products, such as Moringa oleifera leaf extract, has not been fully explored. The optimal concentration of the extract for improving disease resistance is unknown.
Effect of Moringa oleifera Leaf Extract on the Growth Performance, Hematology, Innate Immunity, and Disease Resistance of Nile Tilapia (Oreochromis niloticus) against Streptococcus agalactiae Biotype 2 · 2024 · DOIThe need for research into innovative biomass conversion technologies that can enable more sustainable processes. The gap in the literature regarding the comparison of microwave-assisted and conventional extraction techniques in oil processing.
Microwave-Assisted vs. Conventional Extraction of Moringa oleifera Seed Oil: Process Optimization and Efficiency Comparison · 2024 · DOIThere is a need to analyze the effect of different temperatures on the extraction of oil from moringa seeds. The study aims to fill this gap by providing a comprehensive analysis of the properties of moringa oil extracted at different temperatures.
Fatty Acid Profile and Physicochemical Properties of Moringa oleifera Seed Oil Extracted at Different Temperatures · 2024 · DOIThe degradation of bioactive compounds during digestion is a challenge. The need to protect these compounds to prevent their degradation during digestion is a challenge.
In Vitro Antioxidant, Anti-Inflammatory Activity and Bioaccessibility of Ethanolic Extracts from Mexican Moringa oleifera Leaf · 2024 · DOIThere is a need to explore novel therapeutic avenues to combat bacterial infections. The paper identifies a gap in the use of nanotechnology to address bacterial infections and drug resistance.
Synthesis of Tellurium Nanoparticles Using Moringa oleifera Extract, and Their Antibacterial and Antibiofilm Effects against Bacterial Pathogens · 2024 · DOIThe study does not explicitly state the research gap, but it implies a lack of knowledge on the effects of Moringa oleifera leaf powder on Ningdu yellow chickens. The study aims to fill this gap by investigating the impact of MOLP on growth performance, digestive enzyme activity, meat quality, and cecum microbiota.
Effect of Moringa oleifera Leaf Powder Supplementation on Growth Performance, Digestive Enzyme Activity, Meat Quality, and Cecum Microbiota of Ningdu Yellow Chickens · 2024 · DOIThis improvement may be linked to improved antioxidative defense and decreased oxidative damage, resulting in improved photosynthetic activity and plant biomass; however, the mechanistic validation was not investigated in this study.
Combined Effect of Moringa Extract and Sodium Nitroprusside on the Physiological and Biochemical responses of Narcissus tazetta under Water Deficit Stress · 2026 · DOIFuture research should focus on chronic toxicity, clinical trials, and isolating bioactive compounds to enhance therapeutic benefits while mitigating toxicity risks.
Exploring the dual benefits: acute and subacute toxicity, hypolipidemic, slimming and hypoglycemic effects of Moringa oleifera lam · 2026 · DOIFuture research should focus on standardizing extraction methods, elucidating underlying molecular mechanisms, and conducting well-designed clinical trials to validate efficacy and safety.
Moringa oleifera Lam.: A Nutritional Powerhouse with Multifaceted Pharmacological and Functional Applications · 2025 · DOIFuture investigations integrating metabolomics and transcriptomics are warranted to dissect the molecular mechanisms underlying phytogenic feed additive-mediated microbiota–host crosstalk, particularly its role in regulating key pathways.
Effects of Phytogenic Feed Additive on Production Performance, Slaughtering Performance, Meat Quality, and Intestinal Flora of White-Feathered Broilers · 2025 · DOIAlthough our study does not provide direct evidence of such interactions, these findings highlight the need for further research into the role of viral communities in shaping the rumen microbiome.
Metatranscriptome Analysis of Sheep Rumen Reveals Methane Production Changes Induced by Moringa oleifera as a Dietary Supplement · 2025 · DOIFor this purpose, further research on the synergy between Moringa oleifera and traditional antibiotics is necessary.
Metabolic Profile, Antioxidant, Antimicrobial, Contractile, and Anti-Inflammatory Potential of Moringa oleifera Leaves (India) · 2025 · DOIMoringa oleifera used in traditional medicine to treat bacterial infection has shown considerable antibacterial activity in several in vitro studies but has not been extensively investigated in vivo.
In Vivo Antibacterial and Biochemical Effects of Moringa oleifera Leaf Extract in a Rat Model of Multidrug‐Resistant Salmonella Typhimurium · 2025 · DOIRegardless of the contrary concept, we considered MO a promising plant that could be studied for its medicinal applications on both biochemical and molecular levels.
A growing body of scientific literature provides compelling evidence for the positive impact of AMF on plant growth, especially under challenging environmental conditions. When it comes to combating salinity stress, AMF act as a lifeline for moringa. They form an intricate hyphae network extending far beyond the plant’s root system, effectively increasing the surface area for absorption.
Alleviating Plant Density and Salinity Stress in Moringa oleifera Using Arbuscular Mycorrhizal Fungi: A Review · 2025 · DOIMO represents a sustainable and multi-functional solution to global health challenges, warranting further exploration and integration into clinical and non-clinical applications.
Unveiling the Miracle Tree: Therapeutic Potential of Moringa oleifera in Chronic Disease Management and Beyond · 2025 · DOIMaintaining homeostasis in the intestinal epithelium - Impairment in IEC function due to disorders like inflammatory bowel disease - The need to promote IEC migration and proliferation as a therapeutic…
The Anti-Inflammatory Properties of Polysaccharides Extracted from Moringa oleifera Leaves on IEC6 Cells Stimulated with Lipopolysaccharide In Vitro · 2024 · DOIThe study only investigated the antioxidant and anti-inflammatory properties of Moringa oleifera, - The study did not investigate the effects of Moringa oleifera in vivo, - The study used a limited number of cell lines…
In Vitro Antioxidant, Anti-Inflammatory Activity and Bioaccessibility of Ethanolic Extracts from Mexican Moringa oleifera Leaf · 2024 · DOIOptimize the plant dosage for the beneficial use on other species in aquaculture…
Effect of ethanolic extract of Moringa oleifera leaf on plasma biochemistry and histopathological indices of Heterobranchus bidorsalis Broodstock (Geoffroy ST Hilaire, 1809) · 2026 · DOIWe recommended further research on the molecular effects of MO in healthy and diseased models.
Most-cited papers in Moringa oleifera research and applications
- Deep eutectic solvent-based ultrasonic-assisted extraction of phenolic compounds from Moringa oleifera L. leaves: Optimization, comparison and antioxidant activity · Separation and Purification Technology · 2020 · 294 citations
- Moringa oleifera is a Prominent Source of Nutrients with Potential Health Benefits · International Journal of Food Science · 2021 · 232 citations
- Recent Advances in Drumstick (Moringa oleifera) Leaves Bioactive Compounds: Composition, Health Benefits, Bioaccessibility, and Dietary Applications · Antioxidants · 2022 · 207 citations
- Evaluation of phytochemical and medicinal properties of Moringa (Moringa oleifera) as a potential functional food · South African Journal of Botany · 2020 · 181 citations
- Comparative study on nutrient composition, phytochemical, and functional characteristics of raw, germinated, and fermented Moringa oleifera seed flour · Food Science & Nutrition · 2013 · 127 citations
- A comprehensive review on Moringa oleifera nanoparticles: importance of polyphenols in nanoparticle synthesis, nanoparticle efficacy and their applications · Journal of Nanobiotechnology · 2024 · 124 citations
- Effects of age and extraction solvent on phytochemical content and antioxidant activity of fresh Moringa oleifera L. leaves · Food Science & Nutrition · 2018 · 121 citations
- Treatments and uses of Moringa oleifera seeds in human nutrition: A review · Food Science & Nutrition · 2019 · 92 citations
- Drying alters the phenolic constituents, antioxidant properties, α‐amylase, and α‐glucosidase inhibitory properties of Moringa (Moringa oleifera) leaf · Food Science & Nutrition · 2018 · 86 citations
- Formulation and nutritional evaluation of a healthy vegetable soup powder supplemented with soy flour, mushroom, and moringa leaf · Food Science & Nutrition · 2017 · 78 citations
Most recent work
- Enhanced cytotoxic activity of Moringa oleifera -loaded pharmacosomes against neuroblastoma · Nanoscale Advances · 2026
- Integrative metabolomics using untargeted UHPLC-MS/MS and chemometrics identifies optimal maturity stage of Moringa oleifera leaves from Kuala Terengganu, Malaysia · PeerJ · 2026
- Extraction techniques, structural features, biological functions and bibliometrics of Moringa oleifera Lam. polysaccharides: an updated review · Journal of Ethnopharmacology · 2026
- Nutritional enhancement, antinutrient reduction, sensory quality, and acrylamide mitigation in biscuits fortified with oil-assisted debittered Moringa oleifera leaf powder · Future Foods · 2026
- Phytochemical profiling of gum Moringa oleifera by GC–MS/LC–MS coupled with molecular docking and bioactivity assessment · South African Journal of Botany · 2026
- Green Synthesis of Copper Oxide Nanoparticles by Hydroethanolic Extract of Rubus ellipticus: Antimicrobial and Antidiabetic Activities · Current Nanomedicine · 2026
- Antimicrobial Properties and GC-MS Profiling of Hexane-Derived Fractions of Moringa oleifera Seed Obtained Using Ultrasonic Assisted Extraction Method · UMYU Scientifica · 2026
- Rhizospheric microbiomes as reservoirs for multifaceted agricultural, environmental, and industrial applications · Biomass and Bioenergy · 2026
- Effect of ethanolic extract of Moringa oleifera leaf on plasma biochemistry and histopathological indices of Heterobranchus bidorsalis Broodstock (Geoffroy ST Hilaire, 1809) · Aquaculture International · 2026
- Fungal-Assisted Extraction-SSF of Phenolic Compounds from Moringa oleifera and Its Effects on Antimicrobial and Antioxidant Properties · Fermentation · 2026
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