Open research questions in Mosquito-borne diseases and control
1,849 gap statements mined from Mosquito-borne diseases and control papers in our 7.9M-paper local library, which holds 4,721 papers on the topic — drawn mostly from each paper's own stated research gap, future-work, challenge and limitation notes, and its abstract. The ones listed below are a selection still marked open; each names the study that raised it, with a DOI link where the paper has one.
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What the literature leaves open
Early diagnosis of dengue fever is challenging where confirmatory testing is limited. The study faces the challenge of dealing with an imbalanced dataset. The study needs to optimize the decision threshold for the models, as the default classification threshold of 0.50 may not be optimal.
Tree-Based Machine Learning for Diagnostic Classification of Dengue Fever Using Routine Hematological Parameters: A Secondary Analysis of a Publicly Available Dataset · 2026 · DOIThe epidemiological landscape of DENV in Vietnam is currently undergoing a profound transition. The study aims to fill the gap in understanding the genetic patterns of the 2023 outbreak and the co-circulation of multiple DENV-1 and DENV-2 genotypes. The study investigates the transition toward a more complex evolutionary landscape.
Heterogeneous co-circulation of multiple DENV-1 and DENV-2 genotypes in Vung Tau during the 2023 dengue outbreak in Vietnam · 2026 · DOIOne of the challenges is the complexity of the immune response to Zika virus, which involves multiple cell types and signaling pathways. Another challenge is the limited understanding of the role of Vitamin D in shaping macrophage responses to Zika virus. The analysis of RNA-seq and miRNA-seq datasets also poses technical challenges, such as the need for robust bioinformatic tools and expertise.
Limited development of selective inhibitors due to structural similarities between viral and host proteins. Need for careful target validation to distinguish therapeutically exploitable proviral dependencies from host functions essential for normal cellular and organ physiology.
Antiviral Strategies Against Dengue Virus: Viral Targets, Host Pathways, and Chinese Herbal Medicines · 2026 · DOIThe clinical diagnosis of dengue was delayed due to early positive malaria rapid tests. The patient's symptoms worsened due to the co-infection. The lack of availability of certain diagnostic tests, such as NS1 antigen testing, posed a challenge.
A Case of Intercurrent Dengue and Probable Relapsing Plasmodium vivax Malaria in a Returned Traveler to India: Case Report and Literature Review · 2025 · DOIThe study does not explicitly identify specific technical or domain challenges, but notes that human activities and climate change have expanded the distribution of many vectors, and that there is a need to incorporate seasonal and urban ecological variation into vector management strategies.
Sex Ratio Distortion of Aedes aegypti (L.) in El Salvador: Biocontrol Implications for Seasonally Dry Urban Neotropical Environments · 2025 · DOIThe phenomenon of sex ratio deviation has not previously been demonstrated in container-breeding mosquitoes like A. aegypti. There is a need to incorporate seasonal and urban ecological variation into vector management strategies.
Sex Ratio Distortion of Aedes aegypti (L.) in El Salvador: Biocontrol Implications for Seasonally Dry Urban Neotropical Environments · 2025 · DOIExisting serological assays are limited in their specificity, sensitivity, and cross-reactivity. There is a need for more accurate assays for tracking Dengue virus infection history and guiding vaccination strategies. The cross-reactivity of DENV NS1 antigens is not well understood.
Sensitivity and Cross-Reactivity Analysis of Serotype-Specific Anti-NS1 Serological Assays for Dengue Virus Using Optical Modulation Biosensing · 2025 · DOIThere is a lack of understanding of the biting hour and host-seeking behavior of Aedes species in urban settings. The study aims to fill this gap by providing new insights into the behavior of Ae.
Biting Hour and Host Seeking Behavior of Aedes Species in Urban Settings, Metema District, Northwest Ethiopia · 2025 · DOIIt also highlights the need to evaluate the effectiveness of this approach in a region with high dengue transmission intensity.
Long-Term Durability and Public Health Impact of City-Wide wMel Wolbachia Mosquito Releases in Niterói, Brazil, During a Dengue Epidemic Surge · 2025 · DOIThere is a lack of understanding about the desiccation tolerance of Aedes aegypti and Aedes albopictus eggs in subtropical regions. The study aims to fill this gap by investigating the effect of relative humidity on egg survival.
Desiccation Tolerance of Aedes aegypti and Aedes albopictus Eggs of Northeastern Argentina Origin · 2025 · DOILimited information is available regarding the role of Sabethes spp.
Yellow Fever Virus (YFV) Detection in Different Species of Culicids Collected During an Outbreak in Southeastern Brazil, 2016–2019 · 2025 · DOILimited information is available regarding the role of Sabethes spp. in YFV transmission in the region. The potential for virus reurbanization due to the detection of YFV in other Aedes species is a concern.
Yellow Fever Virus (YFV) Detection in Different Species of Culicids Collected During an Outbreak in Southeastern Brazil, 2016–2019 · 2025 · DOILimited availability of laboratory-confirmed data on arboviral infections in Bangladesh. Difficulty in distinguishing true infections from cross-reactivity, particularly for Zika virus.
Seroprevalence of Dengue, Chikungunya, and Zika Viruses Among Febrile Patients in Dhaka, Bangladesh: A Hospital-Based Cross-Sectional Study · 2025 · DOIFurther research could investigate the use of additional predictor variables, - Further research could explore the application of tree-based algorithms to other diseases, - Further research could examine the performance of tree-based algorithms in different…
Tree-Based Machine Learning for Diagnostic Classification of Dengue Fever Using Routine Hematological Parameters: A Secondary Analysis of a Publicly Available Dataset · 2026 · DOIFuture studies integrating genomic, structural, and functional analyses will be necessary to clarify the significance of substitutions, - Continued diversification is needed to determine whether changes influence viral fitness, transmission efficiency, immune recognition, or other biologically relevant…
Heterogeneous co-circulation of multiple DENV-1 and DENV-2 genotypes in Vung Tau during the 2023 dengue outbreak in Vietnam · 2026 · DOIThe burden of required paperwork for dengue-specific polymerase chain reaction (PCR) - NS1 antigen testing for dengue is not available - Lack of quantitative antibody testing…
A Case of Intercurrent Dengue and Probable Relapsing Plasmodium vivax Malaria in a Returned Traveler to India: Case Report and Literature Review · 2025 · DOIThe study did not aim to establish etiologic causes of illness, - Due to unavailability of a Chikungunya IgM testing kit, only IgG antibody could be tested, - The study had a limited timeframe and relied on convenience…
Seroprevalence of Dengue, Chikungunya, and Zika Viruses Among Febrile Patients in Dhaka, Bangladesh: A Hospital-Based Cross-Sectional Study · 2025 · DOIThe study only considered three sampling points, - The sampling period was limited to May 2022 to December 2023, - The study did not consider other environmental factors that may influence mosquito…
Assessment of the Variation in Faunistic Parameters of Mosquitoes (Culicidae: Diptera) Across Different Forest Gradients in the Tijuca National Park Area, Rio de Janeiro, Brazil · 2025 · DOIFurther studies should investigate the effects of forest fragmentation on mosquito ecology, - Research should focus on developing effective vector control strategies, - Studies should adopt a gradient approach to understand the effects of forest fragmentation on mosquito…
Assessment of the Variation in Faunistic Parameters of Mosquitoes (Culicidae: Diptera) Across Different Forest Gradients in the Tijuca National Park Area, Rio de Janeiro, Brazil · 2025 · DOIOngoing efforts should aim to minimize cross-reactivity while maintaining sensitivity, - Explore integration with complementary platforms for improved diagnostic…
Sensitivity and Cross-Reactivity Analysis of Serotype-Specific Anti-NS1 Serological Assays for Dengue Virus Using Optical Modulation Biosensing · 2025 · DOIThe study did not investigate the detection of other DENV serotypes, - The nanobiosensor had some cross-reactivity with non-complementary targets, - The study was limited to the detection of stDNA…
DNA-Based Nanobiosensor for the Colorimetric Detection of Dengue Virus Serotype 2 Synthetic Target Oligonucleotide · 2025 · DOIDeveloping nanobiosensors for other DENV serotypes, - Investigating the detection of other viruses using the colorimetric detection method, - Improving the specificity…
DNA-Based Nanobiosensor for the Colorimetric Detection of Dengue Virus Serotype 2 Synthetic Target Oligonucleotide · 2025 · DOIFurther evaluation of the devices with a larger and more diverse dataset, - Investigation of the devices' performance with different types of samples, - Comparison of the devices with other diagnostic…
An Improved Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method with Reduced Sample Volume · 2025 · DOIInvestigating the effects of other environmental factors on the survival of Ae.
Desiccation Tolerance of Aedes aegypti and Aedes albopictus Eggs of Northeastern Argentina Origin · 2025 · DOI
Most-cited papers in Mosquito-borne diseases and control
- The global distribution and burden of dengue · Nature · 2013 · 8,313 citations
- Zika Virus Outbreak on Yap Island, Federated States of Micronesia · New England Journal of Medicine · 2009 · 2,529 citations
- Dengue · The Lancet · 2014 · 1,272 citations
- Possible Association Between Zika Virus Infection and Microcephaly — Brazil, 2015 · MMWR Morbidity and Mortality Weekly Report · 2016 · 821 citations
- Efficacy of Wolbachia-Infected Mosquito Deployments for the Control of Dengue · New England Journal of Medicine · 2021 · 624 citations
- RISK FACTORS IN DENGUE SHOCK SYNDROME: A PROSPECTIVE EPIDEMIOLOGIC STUDY IN RAYONG, THAILAND · American Journal of Epidemiology · 1984 · 513 citations
- Climate and Dengue Transmission: Evidence and Implications · Environmental Health Perspectives · 2013 · 496 citations
- Zika: the origin and spread of a mosquito-borne virus · Bulletin of the World Health Organization · 2016 · 401 citations
- Dengue virus: epidemiology, biology, and disease aetiology · Canadian Journal of Microbiology · 2021 · 367 citations
- Recognition determinants of broadly neutralizing human antibodies against dengue viruses · Nature · 2015 · 355 citations
Most recent work
- Review of selected mosquito-borne diseases: arboviruses (dengue, chikungunya, Zika, West Nile, Japanese encephalitis, yellow fever) and parasitic diseases (malaria, lymphatic Filariasis) · Frontiers in Public Health · 2026
- Long-term efficacy and safety of the single-dose tetravalent Butantan dengue vaccine · Nature Medicine · 2026
- Type I IFN autoantibodies underlie chikungunya live-attenuated vaccine encephalitis · Proceedings of the National Academy of Sciences · 2026
- Chikungunya virus persists in joint-associated macrophages and promotes chronic disease in mice · Nature Microbiology · 2026
- West Nile Virus: Epidemiology, Surveillance, and Prophylaxis with a Comparative Insight from Italy and Iran · Vaccines · 2026
- IgA-driven neutrophil activation underlies severe dengue disease after primary Zika virus infection in humans · Nature Immunology · 2026
- The Yellow Fever Vaccine Journey: Milestones and Future Directions · Vaccines · 2026
- Temperature-sensitive incubation, transmissibility and risk of Aedes albopictus -borne chikungunya virus in Europe · Journal of The Royal Society Interface · 2026
- Bundibugyo Virus Disease in 2026 — Clinical and Public Health Responses · New England Journal of Medicine · 2026
- Arboviruses circulation in Guinea: Overview and perspectives for public health · PLoS neglected tropical diseases · 2026
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