Open research questions in Plant Growth Enhancement Techniques
495 gap statements mined from Plant Growth Enhancement Techniques papers in our 7.9M-paper local library, which holds 1,620 papers on the topic — drawn mostly from each paper's own stated research gap, future-work, challenge and limitation notes, and its abstract. The ones listed below are a selection still marked open; each names the study that raised it, with a DOI link where the paper has one.
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What the literature leaves open
Our findings suggest that further studies on the use of NAA in sago palm ex vitro propagation should emphasise varying low concentrations around 1 ppm.
Low NAA concentration appears effective in promoting roots and shoot in sago palm suckers under ex-vitro propagation · 2026 · DOINotably, MPH also induced the accumulation of lactucin and melatonin, bioactive compounds that have been associated with sedative, anti-inflammatory, and sleep-regulatory properties in other contexts; their relevance to human health was not assessed here and warrants further investigation.
LC–HRMS/MS metabolomics reveal biostimulant-mediated nutrient stress alleviation by milk protein hydrolysate in hydroponic Cos lettuce · 2026 · DOIThere is a need to investigate the influence of GA3 on biochemical attributes promoting fruit quality in peach. The optimal concentration of GA3 for improving fruit quality is not well established. The study aims to address this gap by evaluating the effect of GA3 on fruit quality.
Influence of Gibberellic Acid on Biochemical Attributes Promoting Fruit Quality in Peach cv. ‘Shan-i-Punjab’ · 2026 · DOIDespite these advantages, several aspects still limit their full exploitation and require further investigation.
However, despite the increasing interest in using biostimulants in sustainable cropping systems, this field is still underexplored in wild edible greens, which are highly appreciated for their plasticity to unfavorable conditions and are suggested as alternative horticultural crops within the climate change context.
Biostimulatory Effects of Seaweed Extracts and Beneficial Fungi and Bacteria on Crop Performance and Chemical Profile of Sonchus oleraceus, Cichorium spinosum and Scolymus hispanicus · 2026 · DOIFuture research should focus on standardizing field application protocols, integrating advanced omics technologies, and exploring synergistic approaches, such as combining biostimulants with phytoremediation techniques, to enhance agricultural sustainability, food safety, and consumer health in PTEs-contaminated environments.
Harnessing biostimulants to alleviate potentially toxic element stress in vegetable crops · 2026 · DOIHowever, their agronomic effectiveness under realistic agricultural conditions remains to be confirmed through greenhouse and field-scale validation trials, including assessment across different crops, soils, application rates, and environmental conditions.
The lack of exploration of aquatic macrophytes from the Pantanal as sources of plant-derived biostimulants. The need for sustainable and efficient methods for biostimulant production. The importance of characterizing the functional groups of biostimulant extracts.
Bioprospecting Pantanal Aquatic Macrophytes as Sources of Plant-Derived Biostimulants for Soybean Seed Treatment · 2026 · DOILimited understanding of the long-term effects of biostimulants under diverse field conditions.
Harnessing the Power of Biostimulants: A Comprehensive Review of Their Role in Enhancing Agricultural Productivity and Sustainability · 2026 · DOIField results are often inconsistent due to the simultaneous occurrence of multiple abiotic stressors, which are rarely replicated in short-term greenhouse experiments. Responses can vary among cultivars, emphasizing the importance of genotype-specific evaluations.
Integrated Approach of Using Biostimulants for Improving Growth, Physiological Traits, and Tolerance to Abiotic Stressors in Rice and Soybean · 2025 · DOIThe effectiveness of biostimulants remains limited by product heterogeneity, lack of standardized protocols, and strong dependence on cultivar, phenological stage, and local environmental factors. Most research has been conducted in short-term or greenhouse studies, while long-term, multi-year, and field evaluations are still scarce.
Advances in Biostimulant Applications for Grapevine (Vitis vinifera L.): Physiological, Agronomic, and Quality Impacts · 2025 · DOIIn the current study, we changed in the variety PS1296 from previous studies to Heinz 5108, and while they have the same growth behavior and crop cycle, this change might affect the results, which need to be further validated with the actual optimization of the N dose for this specific variety.
A Multifaceted Approach to Optimizing Processed Tomato Production: Investigating the Combined Effects of Biostimulants and Reduced Nitrogen Fertilization · 2025 · DOIThe need for new crop protection products that are environmentally friendly. The lack of efficient alternatives to conventional treatments. The need for a biostimulant seed nano-priming agent for cereals.
Field Crop Evaluation of Polymeric Nanoparticles of Garlic Extract–Chitosan as Biostimulant Seed Nano-Priming in Cereals and Transcriptomic Insights · 2024 · DOIThere is a need to evaluate the effect of different herbicides and biostimulants on soybean crops.
The Effect of Herbicides and Biostimulant Application on the Seed Yield and Seed Quality of Soybean (Glycine max (L.) Merr.) · 2024 · DOIThe effects of biostimulant application on processing tomato and potato yields and quality are not well understood. There is a need for more comprehensive studies on the interaction of biostimulant application with agronomic management.
Processing Tomato and Potato Response to Biostimulant Application in Open Field: An Overview · 2024 · DOIWater scarcity is a significant challenge in agriculture. Climate change and population growth exacerbate the issue. Limited water resources in productive regions like the Mediterranean area pose a challenge for sustainable agriculture.
There is a need to develop strategies to optimize water resource use in agriculture. Current production systems have not fully addressed the issue of water scarcity.
There is a need to improve pepper plant resistance to calcium deficiency and enhance fruit quality.
Effect of Salicylic Acid and Calcium on Growth, Yield, and Fruit Quality of Pepper (Capsicum annuum L.) Grown Hydroponically · 2024 · DOIPoultry manure, ammonium nitrate + patent kali and tree branch chips + poultry manure treatments provided the optimum effects on total and fruit biomass, plant nutrition, photosystem II functionality and fruit quality of Solanum melongena L. plants; in contrast, a significant decline in all the above parameters was found in the CONTROL and tree branch chip-treated plants.
Inorganic and Organic Fertilization Effects on the Growth, Nutrient Uptake, Chlorophyll Fluorescence and Fruit Quality in Solanum melongena L. Plants · 2025 · DOIHowever, the effects of applying green seaweed-based biostimulants, such as SWS or SWE, on the quality of tomato fruits grown under standard conditions have been scarcely documented in the literature.
Productivity and Quality Characteristics of Tomato Fruits (Solanum lycopersicum) Are Improved by the Application of a Green Seaweed (Ulva ohnoi) · 2025 · DOIObserving the current agricultural panorama, the practices carried out in all production systems are dependent on synthetic chemical products, whether to nourish the crops, eradicate weeds, pathogenic fungi, or insect pests. In accordance with this information, brown algae are a viable option to reduce the use of synthetic chemicals that cause deterioration of the environment and in turn that of the plants.
Using Brown Algae in the Plant–Soil System: A Sustainable Approach to Improving the Yield and Quality of Agricultural Crops · 2025 · DOIThe application of biostimulants constitutes an alternative approach toward a more sustainable agriculture that is less reliant on fertilizers and agrochemicals. Those derived from natural sources are of particular interest, especially hPDBs, given their ecological advantages and broad spectrum of action on plant physiology.
Higher Plant-Derived Biostimulants: Mechanisms of Action and Their Role in Mitigating Plant Abiotic Stress · 2024 · DOIThe variable efficacy of biostimulants depending on the specific type and fruit part is highlighted.
Impact of Two Brown Seaweed (Ascophyllum nodosum L.) Biostimulants on the Quantity and Quality of Yield in Cucumber (Cucumis sativus L.) · 2024 · DOIThe study identifies a gap in understanding the impact of fertilizers on crop quality, particularly the effect of different N:K:S ratios on macroelement contents. The experiment aims to fill this gap by investigating the effects of urea and ammonium nitrate solution with potassium thiosulfate on three crop species.
Application of Urea and Ammonium Nitrate Solution with Potassium Thiosulfate as a Factor Determining Macroelement Contents in Plants · 2024 · DOIProposing methods and fostering discussions on INM’s future development, - Exploring modern sustainable soilless production techniques such as hydroponics, aeroponics, and aquaponics, - Investigating the potential benefits of INM for various vegetables and field…
Integrated Nutrient Management of Fruits, Vegetables, and Crops through the Use of Biostimulants, Soilless Cultivation, and Traditional and Modern Approaches—A Mini Review · 2024 · DOI
Most-cited papers in Plant Growth Enhancement Techniques
- Biostimulant Properties of Seaweed Extracts in Plants: Implications towards Sustainable Crop Production · Plants · 2021 · 553 citations
- Chemical Structure and Biological Activity of Humic Substances Define Their Role as Plant Growth Promoters · Molecules · 2021 · 349 citations
- Understanding the Role of Humic Acids on Crop Performance and Soil Health · Frontiers in Agronomy · 2022 · 346 citations
- Successful Plant Growth-Promoting Microbes: Inoculation Methods and Abiotic Factors · Frontiers in Sustainable Food Systems · 2021 · 338 citations
- Flavonoids in Agriculture: Chemistry and Roles in, Biotic and Abiotic Stress Responses, and Microbial Associations · Agronomy · 2020 · 321 citations
- Algae biostimulants: A critical look at microalgal biostimulants for sustainable agricultural practices · Biotechnology Advances · 2021 · 296 citations
- Bacterial Plant Biostimulants: A Sustainable Way towards Improving Growth, Productivity, and Health of Crops · Sustainability · 2021 · 288 citations
- Biostimulants for the Regulation of Reactive Oxygen Species Metabolism in Plants under Abiotic Stress · Cells · 2021 · 267 citations
- Toward a Sustainable Agriculture Through Plant Biostimulants: From Experimental Data to Practical Applications · Agronomy · 2020 · 257 citations
- Algae as Bio-fertilizers: Between current situation and future prospective · Saudi Journal of Biological Sciences · 2022 · 254 citations
Most recent work
- Foliar Stimulants: A Sustainable Strategy for Enhancing Plant Resilience to Abiotic and Biotic Stresses · Physiologia Plantarum · 2026
- Unlocking plant abiotic stress resilience through biostimulants and omics‐driven innovations · Journal of Integrative Plant Biology · 2026
- Harnessing the Power of Biostimulants: A Comprehensive Review of Their Role in Enhancing Agricultural Productivity and Sustainability · Applied Sciences · 2026
- Plant-Derived Biostimulants and Liposomal Formulations in Sustainable Crop Protection and Stress Tolerance · Applied Sciences · 2026
- Exogenous biostimulants: mechanisms and innovations for enhancing seed germination and resilience under abiotic stress · Journal of Advanced Research · 2026
- Seed, leaf, or soil? rethinking agricultural application strategies for boosting crop growth, resilience, and yield · Plant and Soil · 2026
- Structural diversity, biosynthesis, and extraction of brown algae fucoidan and its bio-stimulant applications in crop improvement · Critical Reviews in Biotechnology · 2026
- Enhancing wheat drought resilience through organic amendments: integrating soil health improvement and antioxidant defense activation · BMC Plant Biology · 2026
- Biostimulants Enhance the Growth and Nutritional Quality of Lettuce (Lactuca sativa L.) · Horticulturae · 2026
- Biostimulants and fruit crop performance: Mechanistic insights and agronomic outcomes · Scientia Horticulturae · 2026
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