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Biochemistry, Genetics and Molecular Biology · Research topic

Open research questions in Plant tissue culture and regeneration

64 unresolved questions extracted from the limitations and future-work sections of 663 Plant tissue culture and regeneration papers in our library. Each links back to the study that raised it.

What the literature leaves open

  • Genetic and epigenetic instability associated with prolonged in vitro culture. Freezing injury and desiccation stress during cryopreservation. The need for precise control of explant water content for successful cryopreservation.

    Optimization of the D-Cryoplate Technique for Cryopreservation of <i>Cannabis sativa</i> Axillary Buds · 2026 · DOI

  • Line-dependent differences in morphological characteristics and proliferation. The need for optimized protocols for somatic embryogenesis in sugi. Public health concerns associated with heavy seasonal pollen loads from sugi.

    Temporal morphological characterization of embryogenic cultures of sugi (Cryptomeria japonica, Japanese cedar) · 2026 · DOI

  • Climate volatility and genetic erosion are having a dramatic impact on current wheat production and demand. The need for reliable in vitro plant regeneration from cultured cells and tissues.

    PROTOCOL OPTIMIZATION FOR EMBRYO RESCUE: IMMATURE EMBRYO CULTURE OF WHEAT AND VARIETAL PERFORMANCE ANALYSIS FOR CALLUS INDUCTION · 2026 · DOI

  • There was a need for a reproducible protocol for the genetic transformation of potato cv. Spunta - The use of hygromycin as a selective agent had not been fully explored

    Efficient Transformation of Solanum tuberosum cv. Spunta Mediated by Agrobacterium tumefaciens Using Hygromycin as a Selective Agent · 2026 · DOI

  • The primary bottleneck limiting the acceleration of genetic improvement and the rapid dissemination of superior cultivars is the inherently low multiplication rate of conventional vegetative propagation. There is a need for more effective and efficient methods for cassava propagation.

    Development and evaluation of a synthetic seed system for cassava propagation · 2026 · DOI

  • Further studies can be conducted to analyze the callogenesis process in other plant species. Further studies can be conducted to improve the efficiency of plant tissue culture techniques.

    Dual morphometric and microcellular spatial distribution analysis during zone-specific callogenesis in Fouquieria splendens · 2026 · DOI

  • The study identifies a gap in the understanding of the callogenesis process in Fouquieria splendens. The study aims to provide a better understanding of the cellular architecture of Fouquieria splendens callus.

    Dual morphometric and microcellular spatial distribution analysis during zone-specific callogenesis in Fouquieria splendens · 2026 · DOI

  • Further studies can be conducted to improve the transformation efficiency - Other selective agents can be explored for use in genetic transformation

    Efficient Transformation of Solanum tuberosum cv. Spunta Mediated by Agrobacterium tumefaciens Using Hygromycin as a Selective Agent · 2026 · DOI

  • Although the effects of plant growth regulators (PGRs) on Rose regeneration have been extensively studied, the contribution of growth additives, either alone or in combination with PGRs, remains insufficiently understood in Rosa chinensis.

    Putrescine-Mediated Modulation of Morphological and Reproductive Differentiation During Direct In Vitro Regeneration of Rosa chinensis cv. Old Blush · 2026 · DOI

  • Investigation of the long-term effects of pingyangmycin on litchi plants. Comparison of the effectiveness of pingyangmycin with other mutagens. Application of the pingyangmycin-induced mutagenesis system to other fruit crops.

    Establishment of an in vitro pingyangmycin-induced mutagenesis system for litchi via embryogenic callus regeneration · 2026 · DOI

  • The litchi breeding technology system remains underdeveloped compared with other fruit crops. There is a need for new methods for breeding new litchi varieties with desirable traits. The study aims to address this gap by establishing a pingyangmycin-induced mutagenesis system for litchi.

    Establishment of an in vitro pingyangmycin-induced mutagenesis system for litchi via embryogenic callus regeneration · 2026 · DOI

  • There is a need for improved control of embryo maturation and synchronization of developmental stages. The development of scalable culture platforms is necessary to support high-fidelity clonal propagation. Further research is needed to overcome genotype specificity and asynchronous embryo development.

    Biotechnology of meliaceae: Micropropagation, somatic embryogenesis, and genetic transformation strategies · 2026 · DOI

  • Further studies can investigate the effects of different NAA-BAP combinations on callus induction and morphology in other plant species. The development of new protocols for G. arrostii and other plant species can be informed by the study's findings.

    NAA-BAP Effects on Callus Induction and Morphology in Gypsophila arrostii · 2026 · DOI

  • The study aims to address the lack of knowledge on the effects of NAA-BAP combinations on callus induction and morphology in G. arrostii. The importance of auxin-cytokinin balance in callus induction success is not well understood.

    NAA-BAP Effects on Callus Induction and Morphology in Gypsophila arrostii · 2026 · DOI

  • The limited availability of improved crop varieties in sub-Saharan Africa. The underutilization and weak integration of Bambara groundnut into formal research and market systems.

    In vitro Regeneration of Two Local Bambara Groundnut Landraces [Vigna subterranea (L.) Verdc.] in Kenya · 2026 · DOI

  • Several constraints should be considered. Only two landraces were included, limiting generalization across Bambara germplasm. The hormone regimes covered a defined concentration range; responses outside these windows were not tested. Culture conditions such as light spectrum, carbon source, antioxidants, and subculture intervals were not systematically varied, though they may influence morphogenesis. Indirect regeneration failed, yet physiological or molecular markers of callus competence were not assessed, restricting mechanistic interpretation. In addition, acclimatization was not evaluated, preventing conclusions on ex vitro survival and field performance. These factors indicate directions for refinement rather than weaknesses in experimental execution.

    In vitro Regeneration of Two Local Bambara Groundnut Landraces [Vigna subterranea (L.) Verdc.] in Kenya · 2026 · DOI

  • The rooting rate remained relatively low for effective root induction. The study does not report on the long-term survival and growth of the micropropagated plants.

    Optimised in vitro propagation protocol for the avocado rootstock ‘Duke 7’ ( <i>Persea americana</i> Mill.): Sterilisation, proliferation and rooting · 2026 · DOI

  • The recalcitrant nature of avocado poses significant challenges during micropropagation. The lack of effective and reliable propagation techniques hinders sustainable production in the avocado industry.

    Optimised in vitro propagation protocol for the avocado rootstock ‘Duke 7’ ( <i>Persea americana</i> Mill.): Sterilisation, proliferation and rooting · 2026 · DOI

  • To investigate the effect of different concentrations of TDZ, BAP, and Kin on shoot induction in other hemp varieties. To determine the optimum medium for rooting in other hemp varieties. To develop a protocol for the genetic transformation of C. sativa using the developed regeneration and micropropagation protocol.

    Efficient in vitro Micropropagation of Hemp (Cannabis sativa) genotype · 2026 · DOI

  • The lack of an effective regeneration and micropropagation protocol for the industrial hemp genotype Narlısaray. The need for a safe protocol for in vitro production, rooting, and acclimation of C. sativa.

    Efficient in vitro Micropropagation of Hemp (Cannabis sativa) genotype · 2026 · DOI

  • Further studies can be conducted to optimize the micropropagation protocol for Nepeta baytopii. The protocol can be used for the large-scale production of Nepeta baytopii. The study provides an alternative approach for the propagation of other endangered plant species.

    In vitro micropropagation of the endemic plant Nepeta baytopii Hedge & Lamond · 2026 · DOI

  • There is a need for an effective micropropagation protocol for Nepeta baytopii. Previous studies have reported low rates of germination in vitro for Nepeta species.

    In vitro micropropagation of the endemic plant Nepeta baytopii Hedge & Lamond · 2026 · DOI

  • The limitations of traditionally used callus, including inconsistent secondary metabolite production, slow growth rate, and genetic instability. The need for an alternative to traditional callus culture for the production of valuable phytochemicals.

    Cambial meristematic cells from bitter melon (Momordica charantia L. AMP-06): Propagation, characterization, and kuguacin J content · 2026 · DOI

  • To apply the optimized medium composition to other species of ferns with potential ornamental and medicinal uses. To evaluate the effects of other plant growth regulators on green globular body proliferation and plantlet regeneration.

    Induction of Green Globular Bodies and Plantlet Regeneration in <i>Adiantum trapeziforme</i> · 2026 · DOI

  • Conventional fern breeding relies primarily on sporeling and subsequent selection of sporophytes, which can be time-consuming and labor-intensive. In vitro regeneration via green globular bodies is a potential alternative for breeding and propagation, but the optimal medium composition is unknown.

    Induction of Green Globular Bodies and Plantlet Regeneration in <i>Adiantum trapeziforme</i> · 2026 · DOI

Most-cited papers in Plant tissue culture and regeneration

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64 open questions have been extracted from the limitations and future-work passages of 663 Plant tissue culture and regeneration papers in our library. Each one below links back to the study that raised it, so you can read the original claim in context.

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