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Open research questions in Fungal Biology and Applications

696 gap statements mined from Fungal Biology and Applications papers in our 7.9M-paper local library, which holds 1,694 papers on the topic — drawn mostly from each paper's own stated research gap, future-work, challenge and limitation notes, and its abstract. The ones listed below are a selection still marked open; each names the study that raised it, with a DOI link where the paper has one.

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What the literature leaves open

  • Limited understanding of the effects of Huangshui concentration on Scenedesmus quadricauda growth. Lack of optimization of culture conditions for semi-continuous cultivation of Scenedesmus quadricauda. Need for improved biomass yield in wastewater treatment and bioenergy production.

    Semi-continuous Cultivation of Scenedesmus quadricauda for High Biomass Production Utilizing the Byproduct Huangshui from Chinese Liquor Brewing · 2026 · DOI

  • There is a need for a comprehensive synthesis of the current evidence on Cordyceps and its potential as a therapeutic agent. The traditional notion of Cordyceps has been redefined by recent taxonomic revisions, and there is a need to understand the implications of this redefinition. There is a need to discuss the current evidence on the pharmacological activities of Cordyceps species.

    Cordyceps as a multitarget therapeutic platform: biology, bioactive metabolites, and translational challenges · 2026 · DOI

  • Prior studies have not evaluated the effects of β-glucan, taurine, and L-arginine under hypoxia. There is a lack of cross-comparison between these compounds. The study aims to close this gap by using a shared assay for all three compounds.

    In Vitro Mechanisms of β-Glucan, Taurine, and L-Arginine in CoCl2-Treated H9c2 Cardiomyoblasts and a Pre–Post β-Glucan-Rich Mushroom Extract Study in Athletes · 2026 · DOI

  • Further investigations using purified polysaccharide fractions and detailed structural characterization are needed to clarify the contributions of individual structural features to antioxidant activity.

    The Saccharide Mapping and Biological Activity Analysis of Auricularia heimuer Polysaccharides from Different Origins In Vitro · 2026 · DOI

  • The influence of origin on the antioxidant capacity of AHPs has not been examined. There is a lack of knowledge on the physicochemical properties and biological activities of AHPs from different origins. The study aims to address this gap in knowledge.

    The Saccharide Mapping and Biological Activity Analysis of Auricularia heimuer Polysaccharides from Different Origins In Vitro · 2026 · DOI

  • Limited knowledge on the agronomic traits and bioactive compounds of G. leucocontextum. Need for a comprehensive evaluation of the potential of G. leucocontextum as a new resource for bioactive compound development.

    Screening of a Superior Ganoderma leucocontextum Strain and Evaluation of Its Agronomic Traits and Bioactive Compounds · 2026 · DOI

  • The high degree of polymerization, structural complexity, elevated viscosity, and limited solubility of high-molecular-weight polysaccharides often restrict their processing and application. The need for further research on the specific effects of Auricularia auricula polysaccharides on the respiratory system. The challenge of translating the findings from animal models to human applications.

    Comparative Respiratory Protective Effects of Auricularia auricula Polysaccharide Fractions: From Antioxidant-Dependent Antitussive Effects to Microbiota-Associated Anti-Asthmatic Activity · 2026 · DOI

  • Prunin's bioavailability, solubility, and stability limitations need to be addressed. The paper mentions the need for innovative delivery methods, particularly nanoformulations. The potential for prunin to be used as an adjunctive therapy with conventional treatments needs to be explored.

    Prunin: An Emerging Anticancer Flavonoid · 2025 · DOI

  • Prunin has yet to undergo thorough scientific research. The precise molecular mechanisms of action of prunin remain largely unexplored. The potential of prunin as an anticancer agent has not been fully explored.

    Prunin: An Emerging Anticancer Flavonoid · 2025 · DOI

  • Current methods for managing green mould disease may not be sustainable. There is a need for alternative, chemical-free methods for managing the disease.

    Indigenous Bacterial Endophytes as Sustainable Alternatives for Management of Green Mould Disease in Agaricus bisporus · 2025 · DOI

  • The research requires the use of high-throughput sequencing technology, which can be technically challenging. The study needs to consider the protection of C. macranthos during the sampling process.

    Characteristics of Rhizosphere Soil Fungal Communities of Cypripedium macranthos Sw. at Different Latitudes in Heilongjiang Province · 2025 · DOI

  • There is a need to reduce waste generation in mushroom production. The reuse of Spent Mushroom Substrate is a potential solution to this problem. The study aims to investigate the viability of SMS reuse for P. ostreatus cultivation.

    Evaluation of Reuse of Spent Mushroom Substrate for New Pleurotus ostreatus Crop Cycle · 2025 · DOI

  • The need to balance the nutritional content and antioxidant activity of the mushrooms with the cost and sustainability of the cultivation process.

    Pleurotus ostreatus: Nutritional Enhancement and Antioxidant Activity Improvement Through Cultivation on Spent Mushroom Substrate and Roots of Leafy Vegetables · 2025 · DOI

  • The lack of knowledge on the effects of spent mushroom substrate and roots of leafy vegetables on Pleurotus ostreatus cultivation. The need for sustainable and cost-effective methods for mushroom cultivation. The limited understanding of the relationships between substrate composition, mycelial growth rate, and mushroom quality.

    Pleurotus ostreatus: Nutritional Enhancement and Antioxidant Activity Improvement Through Cultivation on Spent Mushroom Substrate and Roots of Leafy Vegetables · 2025 · DOI

  • The study aims to address this gap by screening fungal isolates for exopolysaccharide production and optimizing medium compositions.

    Exopolysaccharide (EPS) Production by Endophytic and Basidiomycete Fungi · 2025 · DOI

  • Limited understanding of the optimal culture medium components for cordycepin production. Need for stable and efficient production of cordycepin.

    Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered Aspergillus oryzae · 2025 · DOI

  • Limited understanding of the optimal culture medium for cordycepin production by engineered Aspergillus oryzae strains.

    Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered Aspergillus oryzae · 2025 · DOI

  • There is a need to explore alternative substrates to reduce ecological strain and maintain high yields and nutritional quality.

    Utilizing Agrobyproducts: Potential Alternative Substrates for Cultivation of Lentinula edodes · 2025 · DOI

  • Limited understanding of how to optimize P. ostreatus cultivation on various agro-industrial by-products. Need for a predictive model to evaluate the potential of agro-industrial by-products as mushroom substrates.

    Optimizing Pleurotus ostreatus Mushroom Cultivation on Various Agro-Industrial By-Products—Development of a Process Analytical Technology Tool for Predicting Biological Efficiency · 2025 · DOI

  • The need for sustainable alternatives for the reuse of agro-industrial waste. The lack of efficient methods for the production and purification of endoglucanases.

    Purification and Functional Characterization of a New Endoglucanase from Pleurotus djamor PLO13 Produced by Solid-State Fermentation of Agro-Industrial Waste · 2025 · DOI

  • The need for an improved biomass determination method, - The need to optimize the fermentation process to achieve a higher biomass yield, - The lack of a kinetic model of microbial growth for B.

    Adsorbed Carrier Solid-State Fermentation of Beauveria bassiana: Process Optimization and Growth Dynamics Modelization Based on an Improved Biomass Determination Method · 2025 · DOI

  • Whether there are other ginkgolides in the mycelium of C. cicadae - Further development of the biomass of C.

    Application of UHPLC-ESI-MS/MS-Based Metabonomic Techniques to Analyze the Cordyceps cicadae Metabolic Profile Changes to the CO(NH2)2 Response Mechanism in the Process of Ergosterol Synthesis · 2025 · DOI

  • Investigate the effects of AAPs on human respiratory health, - Explore the potential applications of AAPs in functional food, - Study the mechanisms of AAPs on gut…

    Comparative Respiratory Protective Effects of Auricularia auricula Polysaccharide Fractions: From Antioxidant-Dependent Antitussive Effects to Microbiota-Associated Anti-Asthmatic Activity · 2026 · DOI

  • Further exploration of microbial polysaccharides for potential industrial applications, - Investigation of the properties and biological activities of the exopolysaccharides produced by the selected fungal…

    Exopolysaccharide (EPS) Production by Endophytic and Basidiomycete Fungi · 2025 · DOI

  • The low volume of purified compound obtained limited the choices of methods, - The study used a small sample of seeds, - The experiments were conducted in a controlled environment, - The study did not evaluate the long-term effects of the PC…

    Production, Purification, and Application of a Biomolecule with Herbicidal Activity Produced by Fusarium fujikuroi in Submerged Cultivation · 2025 · DOI

Most-cited papers in Fungal Biology and Applications

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Open questions raised by papers listed on this page include: (1) Lack of optimization of culture conditions for semi-continuous cultivation of Scenedesmus quadricauda (“Semi-continuous Cultivation of Scenedesmus quadricauda for High Biomass…”, 2026); (2) There is a need for a comprehensive synthesis of the current evidence on Cordyceps and its potential as a therapeutic agent (“Cordyceps as a multitarget therapeutic platform: biology, bioactive…”, 2026); (3) There is a lack of cross-comparison between these compounds (“In Vitro Mechanisms of β-Glucan, Taurine, and L-Arginine in CoCl2-Treated H9c2…”, 2026); (4) Further investigations using purified polysaccharide fractions and detailed structural characterization are needed to clarify the contributions of individual structural features to antioxidant activity (“The Saccharide Mapping and Biological Activity Analysis of Auricularia heimuer…”, 2026); (5) The precise molecular mechanisms of action of prunin remain largely unexplored (“Prunin: An Emerging Anticancer Flavonoid”, 2025). 696 gap statements have been mined from Fungal Biology and Applications papers in our 7.9M-paper local library, which holds 1,694 papers on the topic; the gaps come from whichever of those papers state one. They are mostly the research gaps the authors state and the papers' abstracts, plus future-work, limitations and challenges passages. The ones listed below are a selection still marked open; each names the study that raised it, with a DOI link where the paper has one, so you can read the original claim in context.

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