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Open research questions in Reproductive biology and impacts on aquatic species

176 gap statements mined from Reproductive biology and impacts on aquatic species papers in our 7.9M-paper local library, which holds 1,055 papers on the topic — drawn mostly from each paper's own stated research gap, future-work, challenge and limitation notes, and its abstract. The ones listed below are a selection still marked open; each names the study that raised it, with a DOI link where the paper has one.

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What the literature leaves open

  • The lack of understanding of the molecular characterization and expression profiling of cyp17 in C. magur. The need to develop sustainable aquaculture practices for C. magur.

    Molecular characterization and expression profiling of cyp17 in the catfish Clarias magur with insights from molecular docking and molecular dynamics simulation · 2026 · DOI

  • The paper identifies a gap in the existing methods for sex reversal in aquaculture, as inert diets are not well accepted by B. splendens larvae. It also highlights the need for an effective and safe strategy for the masculinization of B. splendens.

    A live-feed hormone delivery protocol for the Siamese fighting fish (Betta splendens): Bioencapsulation of 17α-methyltestosterone in Artemia nauplii · 2026 · DOI

  • There is a lack of understanding of the effects of dietary soybean isoflavones on ovarian development and mitochondria function in female M. rosenbergii. The current study aims to fill this knowledge gap by examining the effects of different levels of soybean isoflavones on ovarian development and mitochondrial function.

    Modulatory effects of dietary soybean isoflavones on ovarian development and mitochondria function related indices in female Macrobrachium rosenbergii · 2026 · DOI

  • In fact, GLY exhibits poor bioaccumulation potential due to its physicochemical properties and rapid elimination, with limited evidence for persistent tissue retention in aquatic organisms even after exposure to much higher concentrations.

    Dietary Glyphosate Exposure Disrupts Hepatic and Reproductive Function in Female Zebrafish at Regulatory Safe Levels · 2026 · DOI

  • This pattern suggests that although intersex AGs may attempt to restrain ovarian metabolism through IAG signaling, this regulatory effect is insufficient to suppress ovarian maturation.

    Transcriptome analysis of ovaries and androgenic glands reveals metabolic and immune regulation in intersex Eriocheir sinensis · 2026 · DOI

  • The need to understand the regulation of cyp19a1a by nuclear estrogen receptors. The challenge of characterizing the roles of multiple estrogen receptor paralogs.

    Nuclear estrogen receptors differentially regulate the cyp19a1a promoter in Trachinotus blochii · 2026 · DOI

  • However, given the absence of direct functional assessments and in Vitro acrosome reaction induction experiments in the current investigation, it remains undetermined whether these ultrastructural modifications represent biologically significant changes or merely cryo-induced physical deformations.

    Cryopreservation Protocol Optimization for Penaeus monodon Sperm: Reagent Screening and Parameter Refinement · 2025 · DOI

  • There is a lack of mature male broodstock in hatchery practice for brown-marbled grouper. The insufficient supply of male reproductive gametes limits the growth of brown-marbled grouper in aquaculture production. Few studies have used melatonin as an antioxidant in the semen cryopreservation of different fish species.

    Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm · 2024 · DOI

  • Because this study compared a discrete set of concentrations rather than performing a formal optimization, future work applying response-surface or comparable optimization designs is warranted to define the most effective supplementation levels more precisely.

    Natural Antioxidants Enhance Post-Thaw Sperm Quality and Fertilization Performance in Javaen Barb (Systomus rubripinnis) · 2026 · DOI

  • These findings has provided foundational data for the study of eel sperm biology and could offer scientific support for further research on artificial reproduction in eels.

    Ultrastructural Evidence for Dual Sperm Morphotypes in Hormone-Induced Japanese Eel (Anguilla japonica): Implications for Sperm Maturation · 2026 · DOI

  • These findings further confirmed the uniqueness of the species for Japanese eel, though the relationship between sperm morphological variation and fertilization/hatching remains to be further investigated. This form of eel sperm had not been reported before.

    Ultrastructural Evidence for Dual Sperm Morphotypes in Hormone-Induced Japanese Eel (Anguilla japonica): Implications for Sperm Maturation · 2026 · DOI

  • Although no studies have been conducted on the thickness of chorion layers in channel catfish and blue catfish, our experience in working with microinjection of their embryos indicated that their chorion layers are quite thick.

    Genomic Amplification and Ovary-Specific Expression of Zona Pellucida Genes in Channel Catfish and Blue Catfish · 2026 · DOI

  • The paper does not explicitly state the research gap, but it can be inferred as the lack of detailed information on the early ontogeny of cichlids.

    Early Ontogeny of Cichlids Using Selected Species as Examples · 2024 · DOI

  • The endocrine regulation of testicular and ovarian development during early life in chelonians remains poorly understood, despite major morphophysiological changes occurring in this period.

    Immunolocalization of aromatase and estrogen and androgen receptors in the gonads and mesonephros of Podocnemis expansa during the first year of life · 2026 · DOI

  • Even though protocols tested were insufficient to ensure consistent post-thaw viability, the partial success observed in P1E under MeOH and sucrose combinations provides a relevant experimental basis for future refinement of conservation strategies and contributes to development of ex situ germoplasm conservation approaches for P.

    Cryopreservation toxicity and morphological outcomes in Piaractus brachypomus oocytes and embryos · 2026 · DOI

  • Yet, despite their potency and environmental ubiquity, the effects of androgenic EDCs on immune function and reproductive allocation remain poorly understood.

    Androgenic agricultural pollution suppresses immune function and alters reproductive allocation in eastern mosquitofish · 2026 · DOI

  • Research is limited and has shown mixed results regarding effects of formalin preservation on egg diameter.

    Preservation in formalin variably affects egg size parameters of Walleye · 2025 · DOI

  • However, it is not yet known how well cortisol is preserved in the scale during storage.

    Cortisol in fish scales remains stable during extended periods of storage · 2024 · DOI

  • The results obtained in this study provide a comprehensive perspective that contributes to understanding the reproductive strategy and population dynamics of a species exhibiting trioecy, a rare sexual system that remains poorly understood within the Animalia kingdom.

    Reproductive Characteristics of Hermaphrodites in a Trioecious Marine Mussel: Evidence of a Mixed Mating Strategy · 2026 · DOI

  • The study only used one species of prawn, - The sample size was limited to 600 M.

    Modulatory effects of dietary soybean isoflavones on ovarian development and mitochondria function related indices in female Macrobrachium rosenbergii · 2026 · DOI

  • Sex reversal is widely used in biological breeding, but its cellular and molecular consequences in crustaceans are poorly understood.

    Single-cell atlas reveals NLRP2 associated mitochondrial dysfunction in estrogen induced Macrobrachium nipponense sex reversal · 2026 · DOI

  • Investigate the role of other nuclear estrogen receptors in E2-responsive regulation of cyp19a1a - Examine the regulation of cyp19a1a in other species - Study the effects of E2 on cyp19a1a promoter activity…

    Nuclear estrogen receptors differentially regulate the cyp19a1a promoter in Trachinotus blochii · 2026 · DOI

  • Investigate the effects of heatwave events on other fish species, - Examine the long-term consequences of thermal stress on fish populations, - Develop strategies to mitigate the impacts of climate change…

    The Direct Effects of Climate Change on Tench (Tinca tinca) Sperm Quality under a Real Heatwave Event Scenario · 2024 · DOI

  • Investigate the effects of MT on other fish species, - Test higher concentrations of MT to determine the optimal dose, - Explore the use of MT in combination with other…

    Supplementation of Extender with Melatonin Improves the Motility, Mitochondrial Membrane Potential, and Fertilization Ability of Cryopreserved Brown-Marbled Grouper Sperm · 2024 · DOI

  • Simulation-only study, - Small sample size of 6-7 individual males per group, - Limited to a single species…

    The Direct Effects of Climate Change on Tench (Tinca tinca) Sperm Quality under a Real Heatwave Event Scenario · 2024 · DOI

Most-cited papers in Reproductive biology and impacts on aquatic species

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Open questions raised by papers listed on this page include: (1) The lack of understanding of the molecular characterization and expression profiling of cyp17 in C. magur (“Molecular characterization and expression profiling of cyp17 in the catfish…”, 2026); (2) There is a lack of understanding of the effects of dietary soybean isoflavones on ovarian development and mitochondria function in female M. rosenbergii (“Modulatory effects of dietary soybean isoflavones on ovarian development and…”, 2026); (3) These findings further confirmed the uniqueness of the species for Japanese eel, though the relationship between sperm morphological variation and fertilization/hatching remains to be further investigated (“Ultrastructural Evidence for Dual Sperm Morphotypes in Hormone-Induced Japanese…”, 2026); (4) The endocrine regulation of testicular and ovarian development during early life in chelonians remains poorly understood, despite major morphophysiological changes occurring in this period (“Immunolocalization of aromatase and estrogen and androgen receptors in the…”, 2026); (5) Despite their potency and environmental ubiquity, the effects of androgenic EDCs on immune function and reproductive allocation remain poorly understood (“Androgenic agricultural pollution suppresses immune function and alters…”, 2026). 176 gap statements have been mined from Reproductive biology and impacts on aquatic species papers in our 7.9M-paper local library, which holds 1,055 papers on the topic; the gaps come from whichever of those papers state one. They are mostly the research gaps the authors state and the papers' abstracts, plus future-work, limitations and challenges passages. The ones listed below are a selection still marked open; each names the study that raised it, with a DOI link where the paper has one, so you can read the original claim in context.

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